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纤维素降解细菌的筛选、生物学特性及降解效果

贾辉 陈秀蓉 芦光新 孔雅丽 杨成德

草业学报2016,Vol.25Issue(3):60-66,7.
草业学报2016,Vol.25Issue(3):60-66,7.DOI:10.11686/cyxb2014172

纤维素降解细菌的筛选、生物学特性及降解效果

Isolation of cellulose-degrading bacteria and determination of their degradation activity

贾辉 1陈秀蓉 1芦光新 2孔雅丽 1杨成德1

作者信息

  • 1. 甘肃农业大学草业学院,草业生态系统重点实验室,甘肃 兰州 730070
  • 2. 青海大学农牧学院草业科学系,青海 西宁 810016
  • 折叠

摘要

Abstract

Eight bacterial strains were isolated from soil in the east Qilian Mountains.Of these,four strains had a higher capacity for degradation of plant cellulose when cultured on a CMC-Na plate medium.The growth characteristics of the four strains were studied,and the optimum temperature range of the 4 active strains was 25-30℃,the optimum pH for growth was 5 -8,and the optimum sodium concentration was 4% to 5%.A strain designated X1-2 possessed the highest cellulose degradation ability.Based on morphology,gram stain re-action,and 16S rRNA phylogenetic comparison,this strain was identified as a member of the genus Bacillus (Bacillus sp.).It is a bacterium with strong plant cellulose degradation ability.

关键词

纤维素分解菌/筛选/生物学特性/降解效果

Key words

cellulose-decomposing bacteria/screening/biological characteristics/degradation

引用本文复制引用

贾辉,陈秀蓉,芦光新,孔雅丽,杨成德..纤维素降解细菌的筛选、生物学特性及降解效果[J].草业学报,2016,25(3):60-66,7.

基金项目

国家自然科学基金项目(31460152,41261064)和青海省科技厅项目(2014-ZJ-924Q)资助。 ()

草业学报

OA北大核心CSCDCSTPCD

1004-5759

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