草业学报2016，Vol.25Issue(3)：67-76,10.DOI:10.11686/cyxb2015470
高羊茅在低氮胁迫下的蛋白组学分析
Proteomic analysis of nitrogen stress-responsive proteins in the leaves of tall fescue
摘要
Abstract
In order to thoroughly investigate the protein level changes of tall fescue in low-nitrogen conditions, we analyzed the proteome of the leaves from 30 day old plants exposed to low-nitrogen stress using the iTRAP technique.In total,595 proteins were differentially accumulated (295 up-regulated and 300 down-regulated), which participated in diverse metabolic pathways.According to a strict selection standard,we discovered that the genes related to redox reactions and stresses significantly increased.Physiological and biochemical analysis revealed that the contents of chlorophyll,soluble proteins and free amino acid dramatically decreased,while re-active oxygen-scavenging enzymes such as POD,SOD and GS were highly active.The expression pattern of af-fected genes,detected by real-time fluorescence quantitative RT-PCR,coincided with the proteomic data.No-tably,most of the 14-3-3 family of proteins were enriched by nitrogen shortage,suggesting that these involve the key genes that take part in diverse stresses in tall fescue.In this study,we provide proteomic information for tall fescue subjected to low-nitrogen availability.We also highlight some of the key genes involved and dis-cuss their potential uses.关键词
高羊茅/低氮胁迫/蛋白组学/表达分析Key words
tall fescue/nitrogen stress/proteomic/gene expression引用本文复制引用
李小冬,舒健虹,于二汝,吴佳海,蔡一鸣,王小利..高羊茅在低氮胁迫下的蛋白组学分析[J].草业学报,2016,25(3):67-76,10.基金项目
国家自然基金项目“高羊茅光周期调控基因 FaCONSTANS 特异性表达与生物学功能分析”（31360576）资助。 （）
