草业学报2016，Vol.25Issue(3)：77-85,9.DOI:10.11686/cyxb2015219
我国北方9份旱生－沙生植物蒙古冰草遗传多样性研究
The genetic diversity of 9 populations of dry-desert Agropyron mongolicum collected in northern China
摘要
Abstract
Agropyron mongolicum is one of the dominant species in desert steppe across Eurasia,due primarily to its cold and drought resistance.In this study,we analyzed chromosome polymorphism and DNA polymor-phism in 9 populations of A.mongolicum in northern China.The chromosome number of the 9 populations was 7.Cells were diploid and showed no polymorphism in chromosome ploidy.A total of 138 pairs of wheat SSR primers were amplified and analyzed.A total of 21 primer pairs were amplified with specific fragments. The screening rate of SSR primers was 15.2%.A total of 119 specific bands were amplified:specificity was 5.6 and the polymorphism of DNA was rich.POPGEN 32 software was used to calculate the genetic diversity of the 9 populations of A.mongolicum.Population P8 was found to have the least level of diversity,while P3 had the highest.AMOVA software was used to analyze genetic differentiation,indicating that genetic differ-ences come mainly from individuals in the populations.The UPGMA method was used for a cluster analysis of the 9 populations.When the genetic similarity coefficient is 0.80,the materials tested divided into three groups:P1 -P6 ,P7 -P8 and P9 .This paper lays the foundation for the development and utilization of new vari-eties of A.mongolicum.关键词
蒙古冰草/染色体倍性/SSR/遗传多样性Key words
Agropyron mongolicum/chromosome ploidy/SSR/genetic diversity引用本文复制引用
李晓全,高有汉,刘扬,索培芬,韩冰..我国北方9份旱生－沙生植物蒙古冰草遗传多样性研究[J].草业学报,2016,25(3):77-85,9.基金项目
国家自然科学基金（31060057），中国科学院西部之光“人才培养”项目匹配经费和中国农业科学院草原研究所农业科技创新工程资助。 （）
