草业学报2016,Vol.25Issue(3):234-239,6.DOI:10.11686/cyxb2015374
马唐生防菌厚垣孢镰刀菌 ZC201301的生物学特性研究
The biological characteristics of Digitaria sanguinalis pathogen Fusarium chlamydosporum strain ZC201301
摘要
Abstract
Fusarium chlamydosporum strain ZC201301 showed high herbicidal activity towards Digitaria san-guinalis .In the present study,the ‘single factor at a time’method was used to determine biological character-istics of ZC201301.It was found that the optimal mycelial growth media were potato dextrose agar (PDA)and crabgrass leaf juice (CAJ),and the optimal temperature for mycelia growth ranged from 25 to 30℃.The opti-mal initial pH for culture media was 6-8.Strain ZC201301 can sporulate in a variety of media,but the optimal sporulation medium was CAJ.Mycelial growth did not differ significantly in fluorescent light from that in the dark,but darkness was beneficial for sporulation.The optimal carbon sources for mycelium growth was glu-cose,and the best nitrogen source was NH4 NO3 .Oxygen is essential for mycelial growth,and mycelial growth was inhibited when the liquid/gas ratio was less than 1∶1.The herbicidal activity of strain ZC201301 was sen-sitive to relative humidity,and was significantly decreased when humidity was less than 60%.These results in-dicate the strain ZC201301 is amenable to propagation,which is important for the preparation of bio-control a-gents and for their survival under field conditions.关键词
马唐/厚垣孢镰刀菌/生物除草剂/生物学特性Key words
Digitaria sanguinalis/Fusarium chlamydosporum/bioherbicides/biological characteristics引用本文复制引用
李健,李岩,高兴祥,房锋,李美..马唐生防菌厚垣孢镰刀菌 ZC201301的生物学特性研究[J].草业学报,2016,25(3):234-239,6.基金项目
山东省自然科学基金(ZR2015CQ014)和国家863计划(2011AA10A206)资助。 ()