长春中医药大学学报2016，Vol.32Issue(2)：237-240,4.DOI:10.13463/j.cnki.cczyy.2016.02.006
苦瓜总皂苷对2型糖尿病肾病大鼠肾脏细胞间纤连蛋白和胶原Ⅳ蛋白表达的影响
Total saponins of bitter gourd to fibronectin and collagen Ⅳ in type 2 diabetic nephropathy rat
摘要
Abstract
Objective To investigate the effect of total saponins from bitter to the expression of fibronectin and collagen Ⅳin 2 diabetic nephropathy rats. Methods Male SD rats were randomly divided into normal control group, diabetic nephropathy model group and saponins treatment group(5 mg/kg·d treatment)and left renal resection plus a large dose of streptozocin( STZ)intraperitoneal injection of established animal model,after successful built model, normal control group and model group were given physiological saline by gavage,treatment group give saponins ga-vage,respectively in the 4 weeks,8 weeks half of rats were sacrificed,measuring glucose,24 h urine protein quantita-tive and inflammatory factors;immunohistochemistry,RT-PCR and Western blot detection of renal fibrosis even protein and collagen Ⅳ expression. Results The serum creatinine and serum creatinine in the treatment group were signifi-cantly decreased compared with the model group,the difference was significant(P﹤0. 05);After treatment,Immuno-histochemistry,RT-PCR and Blot Western showed that:compared with the model group,the expression of fibronectin and type Ⅳcollagen protein in the kidney tissue was significantly lower than that in the treatment group( P﹤0 . 05 ). Conclusion Total saponins from bitter gourd can inhibit the expression of fibronectin and type Ⅳ collagen in renal tissue of diabetic nephropathy rats,and improve renal function.关键词
苦瓜总皂苷/2型糖尿病肾病大鼠/纤连蛋白/Ⅳ型胶原Key words
total saponins of bitter gourd/type 2 diabetic nephropathy rats/fibronectin/Ⅳ collagen protein分类
医药卫生引用本文复制引用
陈伟,田鲁,王港,李易..苦瓜总皂苷对2型糖尿病肾病大鼠肾脏细胞间纤连蛋白和胶原Ⅳ蛋白表达的影响[J].长春中医药大学学报,2016,32(2):237-240,4.基金项目
湖北省科技厅面上项目“苦瓜总皂苷对2型糖尿病肾病大鼠的治疗作用”（2013FFA003）。 （）
