长春中医药大学学报2016，Vol.32Issue(2)：266-268,3.DOI:10.13463/j.cnki.cczyy.2016.02.016
千年健寒热药性的实验评价
Evaluating cold and hot characteristic of Homalomena occulta(Lour.) Schott by experimental method
摘要
Abstract
Objective Evaluate the cold and hot characteristic of Homalomena occulta( Lour. )Schott by cellu-lar models. Methods MTT assay was used to investigate the effect of Homalomena occulta( Lour. )Schott on the growth of SGC-7901 in vitro. Morphological changes of SGC-7901 treated with Homalomena occulta( Lour. )Schott were observed through invert microscope. Trypan-blue staining was used to analyze the cytotoxicity of Homalomena oc-culta(Lour. )Schott. Results Homalomena occulta( Lour. )Schott can promote the growth and proliferation of SGC-7901 in the concentration range of 2-5 μg/mL. Morphological observation showed Homalomena occulta( Lour. ) Schott could increase the density of SGC-7901 in the same concentration range. Trypan-blue staining showed Homa-lomena occulta(Lour. )Schott had no cytotoxicity on SGC-7901. Conclusion The results reveal in this work that Homalomena occulta( Lour. )Schott shows a hot or warm characteristic and have no cytotoxicity.关键词
千年健/寒热药性/MTT法/SGC-7901细胞/台盼蓝染色Key words
Homalomena occulta( Lour. )Schott/cold and hot characteristic/MTT assay/SGC-7901/trypan-blue staining分类
医药卫生引用本文复制引用
王征,刘建利,唐志书,宋忠兴..千年健寒热药性的实验评价[J].长春中医药大学学报,2016,32(2):266-268,3.基金项目
国家自然科学基金（20872118）；教育部博士点基金（20070697012）；陕西省重点科技创新团队（2012KCT-20）。 （）
