福建农业学报2016，Vol.31Issue(1)：1-6,6.
O 型口蹄疫病毒 VP1蛋白竞争 ELISA 诊断方法的初步建立
Establishment of Competitive ELISA Diagnostic Method for Foot-and-mouth Disease Based on VP1 Protein in Serotype OVirus
摘要
Abstract
Competitive ELISA was developed for detecting the antibodies against the foot-and-mouth disease virus (FMDV)with the purified recombinant pGEX-6P-1-VP1 protein as the antigen and the rabbit-anti-VP1 serum as the competitive antibody.The positive threshold of the competitive ELISA was calculated using statistical methods.The results showed that (a)the dose of the coating antigen protein was 1ug/ml;(b)the working concentration of the detected serum sample was 1 ∶ 40dilution,and the enzyme-labeled goat anti-rabbit lgG was 1 ∶ 2000;(c)the working titer of specific rabbit serum to N protein was 1 ∶500;and,(d)comparing to the liquid-phase blocking ELISA,the sensitivity and specificity of the competitive ELISA were 91.3% and 95.1%,respectively,and the coincident rate between the two methods was 96.7%.It appeared that this newly developed diagnostic method could be applied for the detection of the serum antibodies against serotype O FMDV with high sensibility,specificity and repeatability.关键词
O 型口蹄疫病毒/结构蛋白 VP1/口蹄疫病毒抗体/竞争 ELISAKey words
foot-and-mouth disease/serotype Ovirus/VP1 protein/antibodies against FMDV/competitive ELISA分类
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何奇松,韦达有,兰宗宝,熊毅,陈磊,颜健华,冯淑萍,黄胜斌,胡巧云,梁晟,易春华,许瑞胜..O 型口蹄疫病毒 VP1蛋白竞争 ELISA 诊断方法的初步建立[J].福建农业学报,2016,31(1):1-6,6.基金项目
广西科技攻关项目 （）
