福建农业学报2016，Vol.31Issue(1)：16-21,6.
油果实中查尔酮合成酶基因 PsCHS 的克隆表达分析及其启动子的分离
Cloning,Expression Analysis and Promoter Isolation of Chalcone-synthase Gene from Fruits of Nane,Prunus salicina Lindl.var.cordata
摘要
Abstract
Chalcone-synthase (CHS,EC 2.3.1.74)is an important enzyme involved inflavonoids synthesis pathway in plants.This study aimed to investigate the gene structure and expression profile of CHS gene in the fruits of nane (Prunus salicina Lindl.var.cordata).A full-length cDNA sequence harboring a CHS gene,named PsCHS ,was successfully separated from a normalized full-length cDNA library of matured nane fruits.The up-stream promoter sequence of PsCHS was separated by genome walking strategy using primer pair designed byPsCHS sequence.The length of PsCHS was 1 442 bp with ORF of 1 176 bp and deduced amino acid of 392 aa.From the prediction by an online software,the promoter sequence of PsCHS have typical structure element TATA-box and CAAT-box, photon-response element, anaerote-induced element, endosperm-related element, MYB-bingding element and hormone-response element,etc.RT-PCR indicated that PsCHS had higher expression level at the earlier stage of development of the fruit,especially 40 d after blossom,then decreased to a lower level at the maturing stage. PsCHS seperated in this study was a member of the CHS gene family.Since CHS is a key enzyme involved in flavonoid biosynthesis pathway,PsCHS might act with a regulatory role in biosynthesis of flavonoids.关键词
查尔酮合成酶/全长 cDNA/序列分析/调控元件/表达分析Key words
Nane (Prunus salicina Lindl.var.cordata)/chalcone synthase/full-length cDNA/sequence analysis/regulatory element/expression level分类
农业科技引用本文复制引用
姜翠翠,王玉珍,叶新福..油果实中查尔酮合成酶基因 PsCHS 的克隆表达分析及其启动子的分离[J].福建农业学报,2016,31(1):16-21,6.基金项目
福建省农业科技重大专项子专题（2013NZ0002-1A-8）；福建省自然科学基金（2015J05058）；福建省科技计划项目---省属公益类科研院所基本科研专项 （）
