福建农业学报2016,Vol.31Issue(1):22-26,5.
樱桃番茄‘秋香’组织培养快繁技术研究
Tissue Culture for Accelerated Propagation of Qiuxiang Cherry Tomato, Lycopersicon esculentum var.cerasiforme
摘要
Abstract
Four methods for disinfecting the tomato explants were first compared on their efficacies prior toapplying the sanitized tender lateral buds from Qiuxiang tomatoes (Lycopersicon esculentum var.cerasiforme ) in the greenhouse for the propagation experiment.The selected disinfection treated the explants in a 10% NaClO solutionfor 20 min,followed by another of 0.1% mercuric chloride with a small amount of Tween 20 for 3 min. Theoptimized culture mediumfor establishing the aseptic lines consisted of MS+BA 0.1 mg·L-1 +IBA 0.1 ·L-1 . Whereas,the medium made of MS+BA 0.1 ·L-1 +IBA 0.5 ·L-1 was found to be suitable for the subculture toyield newhealthy and strong Qiuxiang tomato plantlets.关键词
樱桃番茄/外植体/无菌株系/继代培养Key words
cherry tomato (Lycopersicon esculentum var.cerasiforme)/explant/aseptic lines/subculture分类
农业科技引用本文复制引用
赖呈纯,潘红,孙小彦,黄贤贵,范丽华,谢鸿根..樱桃番茄‘秋香’组织培养快繁技术研究[J].福建农业学报,2016,31(1):22-26,5.基金项目
福建省种业创新与产业化工程项目(2014S1477-1);福建省科技重大专项(2014NZ0002-2);福建省农业科学院设施农业专项科研项目 ()