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樱桃番茄‘秋香’组织培养快繁技术研究

赖呈纯 潘红 孙小彦 黄贤贵 范丽华 谢鸿根

福建农业学报2016,Vol.31Issue(1):22-26,5.
福建农业学报2016,Vol.31Issue(1):22-26,5.

樱桃番茄‘秋香’组织培养快繁技术研究

Tissue Culture for Accelerated Propagation of Qiuxiang Cherry Tomato, Lycopersicon esculentum var.cerasiforme

赖呈纯 1潘红 1孙小彦 1黄贤贵 1范丽华 1谢鸿根1

作者信息

  • 1. 福建省农业科学院农业工程技术研究所,福建 福州 350003
  • 折叠

摘要

Abstract

Four methods for disinfecting the tomato explants were first compared on their efficacies prior toapplying the sanitized tender lateral buds from Qiuxiang tomatoes (Lycopersicon esculentum var.cerasiforme ) in the greenhouse for the propagation experiment.The selected disinfection treated the explants in a 10% NaClO solutionfor 20 min,followed by another of 0.1% mercuric chloride with a small amount of Tween 20 for 3 min. Theoptimized culture mediumfor establishing the aseptic lines consisted of MS+BA 0.1 mg·L-1 +IBA 0.1 ·L-1 . Whereas,the medium made of MS+BA 0.1 ·L-1 +IBA 0.5 ·L-1 was found to be suitable for the subculture toyield newhealthy and strong Qiuxiang tomato plantlets.

关键词

樱桃番茄/外植体/无菌株系/继代培养

Key words

cherry tomato (Lycopersicon esculentum var.cerasiforme)/explant/aseptic lines/subculture

分类

农业科技

引用本文复制引用

赖呈纯,潘红,孙小彦,黄贤贵,范丽华,谢鸿根..樱桃番茄‘秋香’组织培养快繁技术研究[J].福建农业学报,2016,31(1):22-26,5.

基金项目

福建省种业创新与产业化工程项目(2014S1477-1);福建省科技重大专项(2014NZ0002-2);福建省农业科学院设施农业专项科研项目 ()

福建农业学报

OA北大核心CSTPCD

1008-0384

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