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一株红椒软腐病病原菌的分离鉴定及致病性研究

张伟 魏蜜 张少琴 傅本重 王立华 鲁旭东

福建农业学报2016,Vol.31Issue(1):40-45,6.
福建农业学报2016,Vol.31Issue(1):40-45,6.

一株红椒软腐病病原菌的分离鉴定及致病性研究

Isolation,Identification and Pathogenicity of a Soft Rot Pathogen from Sweet Peppers

张伟 1魏蜜 1张少琴 1傅本重 1王立华 1鲁旭东1

作者信息

  • 1. 湖北工程学院生命科学技术学院特色果蔬质量安全控制湖北省重点实验室,湖北 孝感 432000
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摘要

Abstract

A pathogen,HBEU-9,was isolated from the soft rotted samples of sweet peppers in Xiaogan,Hubei.In vitro ,inoculated under the laboratory conditions,it could macerate tissues of sweet peppers.In the field,it infected the peppers with the soft rots.Pathogenicity of HBEU-9 on various vegetables,including green peppers,sweet peppers,lettuce,potatoes,carrots,gourd and cabbages,were examined.The results showed that HBEU-9 was strongly pathogenic toward a wide varieties of vegetables.Based on the morphological characteristics and the 16S rDNA sequence analysis,the pathogen was identified to be Erwinia chrysanthemi (Ech).The antagonism of 7 antibiotics on the pathogen identified gentamicin as the most effective agent among all,even at a relatively low concentration of 60 μg·mL-1 .

关键词

红椒/软腐病/分离鉴定/菊欧文氏菌/致病性

Key words

Sweet pepper/soft rotdisease/isolation and identification/Erwinia chrysanthemi (Ech)/pathogenicity

分类

农业科技

引用本文复制引用

张伟,魏蜜,张少琴,傅本重,王立华,鲁旭东..一株红椒软腐病病原菌的分离鉴定及致病性研究[J].福建农业学报,2016,31(1):40-45,6.

基金项目

湖北省教育厅中青年人才项目(Q20152707);国家自然科学基金(31200488);湖北省重点实验室开放基金重点项目 ()

福建农业学报

OA北大核心CSTPCD

1008-0384

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