福建农业学报2016，Vol.31Issue(1)：70-73,4.
‘闽牧6号’狼尾草组织培养再生体系的建立
Establishment of Tissue Culture Regeneration System for Pennisetum,Minmu No.6
摘要
Abstract
In order to establish an efficient regeneration system,Pennisetum,Minmu No.6 was used as the material to study the effects of various explants,culture media and plant hormones onthe callus induction,subculture and differentiation.The results indicated the lateral bud to be the choice explant,and the NB medium with the addition of 2 mg · L-1 of 2,4-D to yield the maximum induction rate of 89.9%.For the subculture medium,the NB medium containing 2 mg·L-1 of 2,4-D and 1 mg·L-1 of KT was the best for the callus development.The best medium for the differentiation was NB+2 mg·L-1 of 6-BA+0.5 mg·L-1 of NAA,rendering a rate of 75%.The optimal medium for rooting was 1/2 MS.The result appeared to have established a desired tissue culture regeneration system for Minmu No.6,as a model.关键词
‘闽牧 6 号’狼尾草/组织培养/愈伤组织Key words
Pennisetum/Minmu No.6/tissue culture/callus分类
农业科技引用本文复制引用
陈志彤,何水林,黄毅斌,林永生..‘闽牧6号’狼尾草组织培养再生体系的建立[J].福建农业学报,2016,31(1):70-73,4.基金项目
福建省自然科学基金项目（2013J01104）；福建省科技计划项目---省属公益类科研院所基本科研专项（2015R1017-1、2015R1017-5）；福建省财政专项---福建省农业科学院科技创新团队建设项目 （）
