福建农业学报2016，Vol.31Issue(1)：79-83,5.
高粱红曲菌 M-3原生质体制备与再生
Protoplast Formation and Regeneration for M.kaoliang M-3
摘要
Abstract
Protoplast formation and regeneration for M.kaoliang M-3 were optimized by the single factor and orthogonal experiments using 1.0 mol·L-1 (pH 8.0)as the high permeability stabilizing solution,and a mixture of 0.3% cellulase and 0.3% snailase as the cleavaging enzyme.Effects of time,temperature and pH on the hydrolysis process forthe for mationand regeneration of the protoplastwere studied.Based on the results obtained from the single factor experiments,the process was optimized through orthogonal experiments.The results indicated that when the substrate was fermented at pH6.0 and 32℃ for 2 h,3.000 × 106 CFU ·mL-1 could be formed with aregeneration rate of 19.33% (protoplast colonycount=5.80×105 CFU·mL-1 ).关键词
高粱红曲菌 M-3/原生质体/原生质体制备/原生质体再生Key words
M.kaoliang M-3/protoplast/protoplast formation/protoplast regeneration分类
农业科技引用本文复制引用
贾瑞博,周文斌,李燕,钟春梅,刘凯丽,赵慧,刘斌,吕旭聪..高粱红曲菌 M-3原生质体制备与再生[J].福建农业学报,2016,31(1):79-83,5.基金项目
中国博士后科学基金项目（2015M570549）；福建省教育厅科技计划项目（JA14107）；国家科技支撑计划子课题（2014BAD15B01）；福建省科技重大专项 （）
