医学分子生物学杂志2016，Vol.13Issue(2)：90-94,5.DOI:10.3870/j.issn.1672-8009.2016.02.006
MiR-143通过靶控KRAS表达对白血病细胞增殖的影响
MiR-143 Regulates the Proliferation of Leukemia HL-60 Cells by Tar-geting KRAS
陈宏 1陈群 2齐鑫 3苏玉明1
作者信息
- 1. 黑龙江中医药大学附属第一医院儿科 哈尔滨市，150400
- 2. 哈尔滨市阿城区人民医院检验科 哈尔滨市，150300
- 3. 黑龙江中医药大学统计教研室 哈尔滨市，150400
- 折叠
摘要
Abstract
Objective To examine the effect of miR-143 on the proliferation of acute leukemia cells HL-60 by targeting KRAS.Methods Luciferase reporter gene assay was used to detect the tar-geting role of miR-143 to KRAS in HL-60 cells.RT-PCR was used to detect the mRNA expression of miR-143 and KRAS in HL-60 cells.Western blotting was employed to detect the protein expression of KRAS, and MTT method to detect the proliferation of HL-60 cells.Results The expression of miR-143 was significantly decreased and that of KRAS protein significantly increased in HL-60 cells when compared with those in peripheral blood mononuclear cells (PBMNCs) (P<0.01) .MiR-143 could specifically bind to 3'-untranslated region ( UTR ) of KRAS gene.The miR-143 expres-sion was significantly increased, the KRAS protein expression significantly reduced, and cell prolif-eration significantly inhibited in cells transfected with miR-143 mimic, which were significantly re-versed after transfection with miR-143 mimic+KRAS.Conclusion MiR-143 can inhibit the prolif-eration of acute leukemia cells HL-60 by target-controlling KRAS.关键词
miR-143/KRAS/白血病/靶控/细胞增殖Key words
miR-143/KRAS/leukemia/cell proliferation/target-controlled分类
医药卫生引用本文复制引用
陈宏,陈群,齐鑫,苏玉明..MiR-143通过靶控KRAS表达对白血病细胞增殖的影响[J].医学分子生物学杂志,2016,13(2):90-94,5.
