军事医学2016，Vol.40Issue(3)：202-206,5.DOI:10.7644/j.issn.1674-9960.2016.03.010
基于荧光碳点的免疫荧光探针构建及其对大肠杆菌的特异性识别
Immunofluorescence probe based on fluorescent carbon dots and its application to the special recognition of Escherichia coli
摘要
Abstract
Objective Carbon dots (CDs) are an emerging carbon nano-material which is environmentally-friendly, economical , efficient and stable .Their fluorescence properties can match the semiconductor quantum dot .Moreover , CDs have more excellent biocompatibilities .The purpose of this experiment is to apply CDs to the fluorescent immune probe to make them a new label , which can replace the traditional fluorescent dyes .Methods Using microwave heating method , the high strength fluorescent carbon dots were prepared .Wtih the EDC coupling method , the high strength fluorescent car-bon dots could bond with Escherichia coli antibodies to form a complex immune fluorescent probe .Specific recognition exper-iments were carried out in the model of E.coli O157∶H7.Results CDs were successfully applied to immune recognition of E.coli O157∶H7 and multicolor fluorescence was observed .Conclusion CDs can serve as a label of the fluorescent im-mune probe , and are expected to become a new type of low toxicity biosensor with independent intellectual property rights .关键词
荧光抗体技术/免疫荧光探针/碳点/特异性/大肠杆菌O157∶H7/纳米结构Key words
fluorescent antibody technique/immunofluorescent probes/carbon dots/specificity/Escherichia coli O157∶H7/nanostructures分类
通用工业技术引用本文复制引用
车望远,杨焜,李钒,刘长军,田丰..基于荧光碳点的免疫荧光探针构建及其对大肠杆菌的特异性识别[J].军事医学,2016,40(3):202-206,5.基金项目
国家自然科学基金（51303210）；天津市自然科学基金（14JCYBJC29500）；天津市科技支撑计划重点项目 （）
