军事医学2016，Vol.40Issue(3)：207-212,233,7.DOI:10.7644/j.issn.1674-9960.2016.03.011
云南一种新型蝙蝠博卡病毒的鉴定与分析
Detection and identification of a new bat bocavirus species in Yunnan
摘要
Abstract
Objective To discover more novel bat viruses and molecularly characterize bat-borne bocavirus diversity in Yunnan.Methods Twenty-six Aselliscus stoliczkanus were sampled in Jinghong , Yunnan, and subjected to viral metagenomic analysis.Specific PCR was used to detect any bocavirus in these samples based on the metagenomic result , while full genome was amplified and compared with other bocaviruses .Results and Conclusion Totally, 3 of the 26 (11.5%) bats were positive for bocavirus, the full genome of which contained 5203 nucleotides and could encode NS1, NP and VP1/VP2 proteins.Phylogenetic analysis showed that this virus shared up to 58.7% and 53.3% amino acid identities with canine bocavirus 1 and canine minute virus .According to ICTV criteria (85%amino acid sequence identity ) on a new species of bocavirus , this virus could be a novel species within genus Bocaparvovirus .This study provides important data to better understand viral diversity in bats and to uncover the relationship between bocavirus and its hosts .关键词
云南/蝙蝠/博卡病毒/进化分析Key words
Yunnan/bats/bocavirus/phylogenetic analysis分类
农业科技引用本文复制引用
李兴宇,涂长春,胡挺松,何彪,陈刚,夏乐乐,周卫国,徐琳,余静,邱薇,张富强,范泉水..云南一种新型蝙蝠博卡病毒的鉴定与分析[J].军事医学,2016,40(3):207-212,233,7.基金项目
国家自然科学基金－云南省联合基金资助项目（U1036601）；国家自然科学基金资助项目 （）
