军事医学2016，Vol.40Issue(3)：213-216,4.DOI:10.7644/j.issn.1674-9960.2016.03.012
Ro60表达下调抑制肺腺癌A549细胞迁移和侵袭
Downregulation of Ro60 inhibits migration and invasion of human lung adenocarci-noma A549 cells
摘要
Abstract
Objective To study the effect of Ro60 on migration and invasion of lung adenocarcinoma A 549 cells. Methods We knocked down Ro60 and analyzed the ability of migration and invasion of A 549 cells.Quantitative real time ( RT)-PCR and Western blotting were performed to detect mRNA and protein levels of selected molecules associated with migration and invasion of neoplasm .Results When Ro60 was downregulated , the migration and invasion of A 549 cells decreased markedly.Meanwhile,the mRNA expression of matrix metalloproteinase (MMP)9,c-Src etc was observably reduced.Furthermore, downregulation of Ro60 diminished the expression of Src protein and the activation of MMP-9 protein.Conclusion Downregulation of Ro60 inhibits migration and invasion of A549 cells by regulating Src protein and activating MMP-9 protein.关键词
Ro60/肺腺癌细胞/迁移/侵袭Key words
Ro60/lung adenocarcinoma/migration/invasion分类
医药卫生引用本文复制引用
姚婉,刘继来,孙鹏达,田宝磊,刘斌,宋宜,孙志贤..Ro60表达下调抑制肺腺癌A549细胞迁移和侵袭[J].军事医学,2016,40(3):213-216,4.基金项目
国家自然科学基金资助项目 （）
