生物灾害科学2016，Vol.39Issue(1)：14-19,6.DOI:10.3969/j.issn.20953704.2016.01.002
莲腐败病菌细胞壁降解酶的提取及活性分析
Purification and Characterization of Cell Wall Degrading Enzymes from Fusarium oxysporum f. sp. nelumbicola
摘要
Abstract
Fusarium oxyporum produces an array of cell wall degrading enzymes (CWDEs) that enable them to penetrate and infect the host plant, and the role of degrading enzymes are different in their invasion. In order to know the major CWDEs of rhizome rot of lotus in pathogenic processes, activities of five main CWDEs were measured. The results showed that the highest activities of polygalacturonase (PG) and cellulase (Cx) were 4.89 and 6.38 U/mg, and the maximum activities of polymethylgalacturonase (PMG), polygalacturonic acid transeliminase (PGTE) and pectin methyltranseliminase (PMTE) were 2.60 U/mg, 0.531 U/mg and 0.093 U/mg, respectively. So, the activities of PG and Cx were significantly higher than that of PMG, PGTE and PMTE, which indicated that PG and Cx were the major CWDEs in pathogenic processes.关键词
镰刀菌/细胞壁降解酶/莲腐败病/多聚半乳糖醛酸酶Key words
Fusarium oxyporum/cell wall degrading enzymes (CWDEs)/rhizome rot of lotus/polygala cturonase (PG)分类
农业科技引用本文复制引用
胡长志,孙晓棠,汪和贵,曾雅静,郑兴汶,崔汝强..莲腐败病菌细胞壁降解酶的提取及活性分析[J].生物灾害科学,2016,39(1):14-19,6.基金项目
国家自然科学基金项目（31301620）、江西省科技支撑计划项目（20121BBF60049）和江西省自然科学基金项目 （）
