生物灾害科学2016，Vol.39Issue(1)：20-26,7.DOI:10.3969/j.issn.20953704.2016.01.003
云南榧种子内生真菌的分离鉴定及抑菌活性筛选
Isolation and Identification of Endophytes from Seeds of Torreya yunnanensis and Its Antimicrobial Activity Screening
摘要
Abstract
In this study, the endophytic fungi were isolated and purified from the seeds of T.yunnanensis collected from Weixi County, Yunnan Province. The morphology of the endophytic fungi was identified,and antimicrobial activity was screened. By fungal separation and purification technology, endophytic fungi were isolated initially from the seeds of T.yunnanensis. Morphological analyses of endophytic fungi were conducted by microscopic observation of sporulation structures, and antibacterial activities were screened by filter paper method. Fifty endophytic fungi were isolated and purified. Among them, 13 belonged to Phomopsis, 7 Fusarium, 5 Anthrax, 4 Paecilomyces, 1 Alternaria, 1 Trichoderma, and 19 without spores. Antimicrobial results showed that crude extracts of endophytic fungi had broader inhibitory effects on ten important plant pathogenic fungi. Z41 and Z43 exhibited the best antimicrobial activities.关键词
云南榧/内生菌/分离纯化/活性筛选/滤纸片法Key words
Keywods：Torreya yunnanensis/endophytes/isolation and purification/antimicrobial activity screening/filter diffusion method分类
农业科技引用本文复制引用
李向楠,周稚凡,刘风路,李靖..云南榧种子内生真菌的分离鉴定及抑菌活性筛选[J].生物灾害科学,2016,39(1):20-26,7.基金项目
云南省生物学优势特色重点学科建设项目 （）
