检验医学与临床2016，Vol.13Issue(7)：885-887,3.DOI:10.3969/j.issn.1672-9455.2016.07.009
大鼠心脏移植急性排斥反应与 CD8＋CD28＋T 淋巴细胞钙离子的相关性研究
Correlation between acute rejection in rat heart transplant with CD8+ CD28+ T lymphocyte calcium ion
摘要
Abstract
Objective To explore the correlation between the acute rejection (AR) in rat heart transplant (HT) with extracellular 45Ca2+ uptake rate and intracellular Ca2+ concentration of CD8+ CD28+ Tlymphocytes . Methods The flow cytometry was used to sort the peripheral blood CD8+CD28+ Tlymphocytes on postoperative 1 , 3 ,5 ,7 ,9 ,11 d in the rat control group (T0 ) ,sham operation group (T1 ) and experiment group (T2 ) ,the extracellular 45Ca2+ uptake rate and intracellular Ca2+ concentration were measured ,and then the correlation between extracellular 45Ca2+ uptake rate and intracellular Ca2+ concentration of CD8+ CD28+ Tlymphocytes was analyzed .Results The extracellular 45 Ca2+ uptake rate and intracellular Ca2+ concentration of CD8+ CD28+ Tlymphocytes in the T2 group were significantly increased compared with the T1 group and T0 group ,the difference was statistically significant(P<0 .05);the 45 Ca2+ uptake rate and intracellular Ca2+ concentration of CD8+ CD28+ Tlymphocytes in the T2 group were positively correlated with the degree of AR ,the Spearman correlation coefficients were 0 .494 and 0 .688 respec‐tively(P<0 .01) .Conclusion Peripheral blood extracellular 45Ca2+ uptake rate and intracellular Ca2+ concentration of CD8+CD28+ Tlymphocytes are closely correlated with the AR in rat HT,which is conducive to early monitor AR af ter H T.关键词
CD8+CD28+ T淋巴细胞/心脏移植/急性排斥反应/钙离子Key words
CD8+CD28+ T lymphocytes/heart transplantation/acute rejection/calciumion引用本文复制引用
余春俊,王涛,涂星强,邹小明..大鼠心脏移植急性排斥反应与 CD8＋CD28＋T 淋巴细胞钙离子的相关性研究[J].检验医学与临床,2016,13(7):885-887,3.基金项目
新疆维吾尔自治区科技计划资助项目（201233149）。 （）
