检验医学与临床2016，Vol.13Issue(7)：888-890,3.DOI:10.3969/j.issn.1672-9455.2016.07.010
多重耐药鲍曼不动杆菌中耐消毒剂基因 qacE △1的存在现状及其阳性菌株的同源性分析
Existing status of disinfectant resistant gene qacE△1 in multidrug-resistant Acinetobacter Bauman and analysis of posi-tive strain homology
摘要
Abstract
Objective To explore and analyze the existing status of disinfectant resistant gene qacE△1 in mul‐tidrug‐resistant Acinetobacter Bauman and its positive strain homology .Methods The disinfectant resistant qacE△1 gene in 80 strains of multidrug‐resistant Acinetobacter baumannii was analyzed by the polymerase chain reaction (PCR) and sequence analysis method ,then the positive strain homology was analyzed by the sequence repeat poly‐merase chain reaction .Results In this study ,42 strains were positive in 80 strains of multidrug‐resistant Acineto‐bacter baumannii ,the positive rate was 52 .5% ,in which 30 strains were type A ,5 strains were type B ,3 strains were type C ,2 strains were type D and 2 strains were type E .The type A was the main epidemic type and accounted for 71 .43% .Among 42 strains of positive multiple drug resistant Acinetobacter Bauman ,the heart surgery department had 8 strains(19% ) ,neurosurgery department had 7 strains(17% ) and central ICU had 6 strains (14% ) .When M IC was 300 mg/L ,the inhibited strains number was maximal and when MIC was 500mg/L ,the proportion of positive strains among inhibited strains was highest(83 .33% ) .Conclusion The drug resistance enhancement of multidrug‐resistant Acinetobacter baumannii has a correlation with disinfectant resistant gene qacE△1 ,moreover five subtypes exist in clinic ,which is mainly the type A .The cardiothoracic surgery department ,neurosurgery department and cen‐tral ICU should be as the main infection control objects .关键词
多重耐药鲍曼不动杆菌/耐消毒剂基因/阳性菌株/同源性Key words
multidrug-resistant Acinetobacter Bauman/disinfectant resistant gene/positive strain/ho-mology引用本文复制引用
张静,张之烽..多重耐药鲍曼不动杆菌中耐消毒剂基因 qacE △1的存在现状及其阳性菌株的同源性分析[J].检验医学与临床,2016,13(7):888-890,3.基金项目
江苏省南京市卫生局2010年医学科技发展基金资助项目（YKK10061）。 （）
