临床口腔医学杂志2016，Vol.32Issue(3)：131-133,134,4.DOI:10.3969/j.issn.1003-1634.2016.03.002
Cal－27舌鳞癌动物模型的建立及其意义
Tongue squamous carcinoma animal model constructed using CAL-27 cell and its significance
摘要
Abstract
Objective:Establishing animal transplanted tumor model of Cal-27 tongue squamous cell carcinoma, studying the biological behavior of tumor cells and metastatic tumor specificity. Method Subcutaneous injecting Cal-27 cell suspension(cell density 1×107 unit/ml,injected dose 0.2 ml)on the left side of the axillary region to establishing trans-planted tumor model. Period measuring tumor's size and keeping the change record of body weight. 4 weeks later,executing all nude mouse,tissue section every mouse,using HE stain to observe the pleomorphism of organizational structure and cell pleomorphism. Examining tumor metastasis in model's lung and liver. Result After the subcutaneous injections,it needs 14 days to catch the tumor formation standard on average. The tumor formation rate is 100%in 24 injected nude mouse. There is no tumor metastasis in model's lung and liver. Conclusion Consider all conditions are appropriate,such as tumor cell u-nit,site of tumor,week-old and status of mouse etc.,the tumor formation rate could be 100 %. Those models objectively re-flect the biological behavior of tongue squamous cell carcinoma. They provide appropriate injection dose for the research of animal models. They also provide ideal animal models for the future research.关键词
Cal-27/舌鳞癌/裸鼠/动物模型Key words
Cal-27/Tongue squamous cell carcinoma/Nude mouse/Animal models分类
医药卫生引用本文复制引用
李娜娜,卢恕来,李培媛,董刚,李涛,杨丕山,郑建金..Cal－27舌鳞癌动物模型的建立及其意义[J].临床口腔医学杂志,2016,32(3):131-133,134,4.基金项目
青岛市科技局重点项目（11－2－3－2－（7）－nsh）青岛市科技局重点项目 （）
