临床口腔医学杂志2016，Vol.32Issue(3)：149-152,153,5.DOI:10.3969/j.issn.1003-1634.2016.03.007
Notch信号通路相关分子在牙周膜干细胞中的表达及意义
Notch signaling pathway related molecules in the expression and significance of the periodontal ligament stem cells
摘要
Abstract
Objective:Detection of Notch signaling pathway related molecules in inflammatory and normal periodon-tal membrane expression differences of cells,and to explore its mechanism in periodontitis alveolar defect. Method:H-PDLSCs and P-PDLSCs were primarily cultured by tissue digestion method. Cells were passaged and identified by stem cell surface marker expression using immunofluorescence staining.Detection of Notch1 receptor proteins and ligand Jagged1 sig-nal molecule expression in the Notch signal pathway by immunofluorescence test and real-time quantitative PCR. Result:The two types of cells both expressed stem cell markers STRO-1 and CD146. P-PDLSCs showed higher multiplication ca-pacity. Both cells all positive expression of Notch1 and Jagged1;The expression of Notch1 mRNA and Jagged1 mRNA in P-PDLSCs were(1.22±0.29),(1.52±0.38)respectively,they were lower than those in H-PDLSCs,the differences were statisti-cally significant (P<0.05). Conclusion:Inflammatory periodontal membrane inflammation of stem cell microenvironment may inhibit the expression of the Notch signaling molecules,the low lenel of the activation of the Notch signal may be good for periodontitis defect alveolar bone ossification of differentiation.关键词
牙周膜干细胞/Notch信号通路/成骨/牙周炎/RT-PCRKey words
Periodontal ligament sem cells/Notch signaling pathway/osteogenesis/Periodontitis/Real time PCR分类
生物科学引用本文复制引用
袁萍,李淑慧,于莉,赵璐,周春梅,吴佩玲..Notch信号通路相关分子在牙周膜干细胞中的表达及意义[J].临床口腔医学杂志,2016,32(3):149-152,153,5.基金项目
国家自然科学基金 （）
