临床口腔医学杂志2016，Vol.32Issue(4)：198-202,5.DOI:10.3969/j.issn.1003-1634.2016.04.003
人脐带间充质干细胞分离培养及鉴定的相关研究
The study of isolatating and identificating the human umbilical cord mesenchymal stem cells
摘要
Abstract
Objective:The aim of this study was to isolate and identify the human umbilical cord mesenchymal stem cells (hUCMSCs). Method:Umbilical cord was cut into pieces, and then digested with collagenaseII. The mesenchymal stem cells from human umbilical cord were cultured. After that, the multipotent differentiation characteristics were demonstrated using standard in vitro conditions. Furthermore,the biological properties of hUCMSCs were investigated by flow cytometry. And the relevant mRNAs levels of Hedgehog signaling pathway were detected by real-time PCR. Result:Monoptychial het-erogeneous cells were obtained.The cells were able to differentiate into osteogenic, adipogenic and cartilage cells. A total of 98.4 % of cells at passage 3 expressed cluster of differentiation CD29 and 98.80% of CD44,85.09% of CD105, but only 0.19%of CD45 and 0.58%of CD34.And expression of the factors of hedgehog signaling pathway were able to be detected by real-time PCR. Conclusion:hUCMSCs that are isolated from the human umbilical cord and exhibit the identified character-istics may be used as seed cells in jaw bone defect reconstruction.关键词
人脐带间充质干细胞/生物学性状/Hh信号通路Key words
Human umbilical cord mesenchymal stem cells/Biological properties/Hedgehog signaling pathway分类
生物科学引用本文复制引用
青莹,黄梦琦,石琦,曹颖光,宋珂..人脐带间充质干细胞分离培养及鉴定的相关研究[J].临床口腔医学杂志,2016,32(4):198-202,5.基金项目
国家自然科学基金（81400490）湖北省自然科学基金 （）
