临床口腔医学杂志2016，Vol.32Issue(4)：203-206,207,5.DOI:10.3969/j.issn.1003-1634.2016.04.004
上皮间质转化相关蛋白在口腔黏膜下纤维化及其癌变组织中的表达
The expression of epithelial-mesenchymal transition associated proteins in Oral Submucous Fibrosis and can-ceration
摘要
Abstract
Objectives:To investigate the expression and significance of E-cadherin and β-catenin protein related with EMT in Oral Submucous Fibrosis(OSF) or the Oral Squamous Cell Carcinoma(OSCC) originating from OSF. Method:The SP immunohistochemistry was used to detect the E-cadherin and β-catenin protein expression in tissues from 60 cases of OSF,15 cases of OSCC originating from OSF and 5 cases of normal oral mucosa,and analyzed its mean optical density (IOD/Area) by IPP image processing software. Result: ①The positive reaction of E-cadherin in immunohistochemical were almost in the cytomembrane. The abnormal expression of E-cadherin protein for the normal,OSF early,moderately ad-vanced,advanced and OSCC were 0,5%,20%,35%and 53.3%respectively,and the mean optical density of E-cadherin were 0.125±0.004,0.116±0.0006,0.109±0.008,0.103±0.009 and 0.095±0.008 respectively.There were significant difference among them (P<0.05).②The positive reaction ofβ-catenin were almost in cytomembrane in the normal control,membrane expression decreased and cytoplasm to nuclear increased in the OSF.The abnormal expression of β-catenin protein for the normal, OSF early,moderately advanced,advanced and OSCC were 0,60 %,80%,90 % and 93.3 % respectively,and the mean optical density ofβ-catenin were 0.105±0.007,0.098±0.009, 0.106±0.012,0.114±0.007 and 0.119±0.003 respective-ly.Though β-catenin decreased in OSF early,there were no significant difference compared with the normal control (P>0.05),whereas there was significant difference among each OSF stage(P<0.05). Conclusion:The expression of E-cadherin decreased while the β-catenin increased in cytoplasmic to nuclear in oral submucous fibrosis and OSCC tissue,EMT possi-bly involved in the process and carcinogenesis of OSF.关键词
口腔黏膜下纤维化/癌变/上皮间质转化/E-cadherin/β-catenin/免疫组化Key words
Oral Submucous Fibrosis(OSF)/canceration/Epithelial-mesenchymal transition/E-cadherin/β-catenin/immunohistochemical分类
医药卫生引用本文复制引用
徐文华,许春姣,周美璐,李冬梅,吴凯悦,池毓坦,王宏峰,孙晓娟..上皮间质转化相关蛋白在口腔黏膜下纤维化及其癌变组织中的表达[J].临床口腔医学杂志,2016,32(4):203-206,207,5.基金项目
湖南省科技计划项目 （）
