临床神经外科杂志2016，Vol.13Issue(2)：110-113,4.DOI:10.3969/j.issn.1672-7770.2016.02.009
荧光实时监测贝伐单抗治疗胶质母细胞移植瘤的实验研究
Bioluminescent imaging of impact of bevacizumab on anti-proliferation and angiogenesis in human glioblastomaxenografts in vivo
摘要
Abstract
Objective To explore the anti-proliferation and angiogenesis effect of bevacizumab (Avastin) on human glioblastomamultiforme (GBM).Methods U373 cells stably transfected with a luciferase expression plasmid ( U373-Luc ) were injected into the brains of RAG-KO mice with a Hamilton syringe under anesthesia.Seven days after inoculation, the mice were randomly divided into 4 groups(6 mice per group).Treatment was initiated after 7 days of tumor cell implantation,according to the following regimens:(1) Control groups receive appropriate vehicles(PBS);(2)bevacizumab;(3) bevacizumab;( 4 ) bevacizumab.Tumor growth was assessed twice a week by bioluminescence imagingusing a XenogenIn Vivo Imaging system.We weighed the mice every 3days and recorded the time until they reached their humane end-points.Three weeks after the treatment, solid tumors from sacrificed mice were OCT-embedded.Expression of CD31were detected by immunohistochemical method.The microvessel density(MVD) were assessed under microscope.ELISA method was used to measure the expression level of the vascular endothelial growth factor ( VEGF ) .Results The expression VEGF and MVD was significantly decreased in both 10 mg/kg and 20 mg/kg treated group when compared to control group and 2.5mg/kg treated group.The growth of tumor in 10 mg/kg and 20 mg/kg treated group remarkably reduced during the initial 2weeks, however,it rebound to quick growth from the third week.Conclusions These findings suggest that bevacizumab can notably inhibited tumor cell proliferation and tumor angiogenesis, but the effects declined with the time oftreatment.Adverse drug reaction was not found in high dose treated group.关键词
胶质母细胞瘤/贝伐单抗/血管生成萤光素酶Key words
glioblastoma/bevacizumab/angiogenesis/luciferase分类
医药卫生引用本文复制引用
刘翔宇,孙康健,王汉东,王娟..荧光实时监测贝伐单抗治疗胶质母细胞移植瘤的实验研究[J].临床神经外科杂志,2016,13(2):110-113,4.基金项目
江苏省医学重点学科基金资助项目( X4200722 ) （ X4200722 ）
南京军区南京总医院院管课题(2011047) （2011047）
