畜牧与饲料科学2016,Vol.37Issue(3):1-3,4,4.
利用JIVET技术快速繁育萨福克羊的研究
Study on Rapid Propagation of Suffolk Sheep by Using JIVET Technology
摘要
Abstract
JIVET is a technique using reproductive hormone to stimulate ovarian of young stock to rapidly and efficiently produce oocytes. In this study, in order to evaluate the application performance of JIVET in rapid propagation of Suffolk Sheep, 4 one-month-old lambs of Suffolk Sheep were selected and intramuscularly administrated with FSH and PMSG. Forty-eight hours after administration of reproductive hormone, the oocytes were collected by surgery and were subsequently matured in vitro. The matured oocytes were fertilized with prepared frozen semen in vitro. When the embryos grew to 2 to 8 cells each, they were transferred into the uterus of 6 receptor female sheep. The results revealed that a total of 109 available oocytes were obtain after the treatment of reproductive hormone and 79 of them were successfully matured in vitro, and the maturation rate was 72.48%. A total of 34 cleaved embryos were obtained after fertilization in vitro and the cleavage rate was 43.04%. Three receptor female sheep was pregnant after receiving embryo transfer and the pregnancy rate was 50%. Finally, 3 lambs of Suffolk Sheep were obtained. These results suggest the fact that the application of JIVET can shorten the breeding cycle of Suffolk Sheep and thus speed up the breeding of Suffolk Sheep.关键词
萨福克羊/JIVET技术/幼羔/生殖激素/胚胎移植Key words
Suffolk Sheep/JIVET/lamb/reproductive hormone/embryo transfer分类
农业科技引用本文复制引用
刘宗正,李和刚,程明,杨培培,赵金山..利用JIVET技术快速繁育萨福克羊的研究[J].畜牧与饲料科学,2016,37(3):1-3,4,4.基金项目
青岛市民生计划项目(13-1-3-88-nsh)。 ()