畜牧与饲料科学2016，Vol.37Issue(3)：4-6,10,4.
不同浓度玻璃化冷冻保护液及冷冻载体对驴卵母细胞成熟率的影响
Effect of Different Vitrificated Cryopreservation Solutions and Loading Devices on in vitro Maturation Rate of Donkey Oocytes
摘要
Abstract
The aim of the present study was to evaluate the effect of different vitrificated cryopreservation solutions and loading devices on in vitro maturation rate of donkey oocytes. Four concentration gradient of solutions (Ⅰ,Ⅱ,Ⅲ,Ⅳ) and 3 sets of loading devices were included in this study. The GV-stage oocytes of donkey were cryopreserved by using different solutions and loading devices and were subsequently matured in vitro after thawing. The maturation rate of donkey oocytes was served as an index to compare their performance in cryopreservation. The results demonstrated that the maturation rate of oocytes cryopreserved by solutionⅡ (28.13%) was significantly higher than that of oocytes cryopreserved by the other 3 solutions (P<0.05), but was significantly lower than that of oocytes in control (51.77%,P<0.05). The maturation rate of oocytes cryopreserved by OPS (28.13%) was significantly higher than that of oocytes cryopreserved by ordinary glass straws (11.67%,P<0.05), but was not significant different compared with that of oocytes cryopreserved by GMP(23.53%,P>0.05).关键词
驴/玻璃化冷冻/卵母细胞/成熟率/载体Key words
donkey/vitrificated cryopreservation/oocytes/maturation rate/loading devices分类
农业科技引用本文复制引用
杜兴雨,王彦妮,张焱如,曹俊伟..不同浓度玻璃化冷冻保护液及冷冻载体对驴卵母细胞成熟率的影响[J].畜牧与饲料科学,2016,37(3):4-6,10,4.基金项目
国家自然科学基金（31460292）。 （）
