畜牧与饲料科学2016，Vol.37Issue(3)：14-17,21,5.
奶牛子宫内膜组织体外培养方法的建立及前列腺素E2和F2α受体分布的研究
Establishment of Cultivation Method of Bovine Endometrial Tissue in vitro and Expression of PGE2 and PGF2α Receptors
摘要
Abstract
Objective] To establish the cultivation method of bovine endometrial tissue in vitro and to detect the expression of the receptors of PGE2 and PGF2αin the cultured endometrium. [Methods] The fresh endometrial tissue samples from bilateral uterine horn of health dairy cows in proestrus were collected and used for suspension cultivation in vitro. The cultured endometrium was histologically identified, and the expression of the receptors of PGE2, including EP1, EP2, EP3(EP3A、EP3B、EP3C、EP3D), EP4, and the receptors of PGE2αFP were evaluated by RT-qPCR. [Results] The histological assessment indicated that the structure of cultured bovine endometrium in vitro was intact, and the morphology of the glands and nuclear of the cells was normal. The expression of all the tested receptors of PGE2 and PGF2αwere found in the cultured samples from bilateral uterine horn of dairy cows in proestrus. The expression of the receptors varied, the highest relative expression was observed in EP4, and the expression of EP1, EP3B and FP was relatively lower. [Conclusion] The in vitro cultivation method of bovine endometrial tissue was successfully established. The expression of the receptors of PGE2 and PGF2αwere found in cultured endometrium, and the relative expression of different types of receptors varied.关键词
前列腺素/PGE2/PGF2α/受体/实时荧光定量PCRKey words
prostaglandin/PGE2/PGF2α/receptor/RT-qPCR分类
农业科技引用本文复制引用
张双翼,毛伟,刘博,曹金山..奶牛子宫内膜组织体外培养方法的建立及前列腺素E2和F2α受体分布的研究[J].畜牧与饲料科学,2016,37(3):14-17,21,5.基金项目
国家自然科学基金项目（31360627）；内蒙古农业大学科技创新团队项目（NDPYTD2013-1）。 （）
