色谱2016，Vol.34Issue(3)：270-278,9.DOI:10.3724/SP.J.1123.2015.11016
高效液相色谱-串联质谱法同时测定保健品及中成药中非法添加的17种壮阳类化学药
Simultaneous determination of 17 aphrodisiac chemical drugs illegally added in health products and Chinese patent medicines by high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method was proposed for the simultaneous determination of seventeen aphrodisiac chemical drugs which were illegally mixed into the health products and Chinese patent medi-cines by high performance liquid chromatography-mass spectrometry ( HPLC-MS/MS ). The extraction,chromatographic conditions and mass spectrometry parameters were optimized in order to separate three groups of isomers. The drugs were extracted with methanol under ultra-sonic condition,separated on an Agilent Extend C18 chromatographic column( 100 mm × 2. 1 mm,3. 5 μm ) with acetonitrile and water ( additional 10 mmol/L ammonium acetate ) as mobile phases under gradient elution at a flow rate of 0. 25 mL/min. The determination was conducted by tandem mass spectrometry in positive ESI mode under multiple reaction monito-ring( MRM)mode. The method made seventeen aphrodisiac chemical drugs as well as the three groups of isomers to get the ideal separation and accurate quantitative. The method was proved to be rapid,selective,sensitive and stable,and it has been applied to the screening and detec-tion of aphrodisiac chemical drugs illegally added in health products and Chinese patent medi-cines. All the tested samples show that the aphrodisiac chemical drugs have a high detection rate in the recent three years. The relevant regulatory authorities should pay more attention to it.关键词
高效液相色谱-串联质谱/壮阳类化学药/保健品/中成药/非法添加Key words
high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)/aphrodisiac chemical drugs/health products/Chinese patent medicines/illegally added分类
化学化工引用本文复制引用
黄芳,吴惠勤,黄晓兰,罗辉泰,朱志鑫,林晓珊,马立果,蒋娅兰..高效液相色谱-串联质谱法同时测定保健品及中成药中非法添加的17种壮阳类化学药[J].色谱,2016,34(3):270-278,9.基金项目
广东省促进科技服务业发展计划项目（2012B040302007）．@@@@Promoting Technology Services Development Plans（ No．2012B040302007）． （）
