色谱2016，Vol.34Issue(3)：321-326,6.DOI:10.3724/SP.J.1123.2015.11025
超临界流体色谱与高效液相色谱分离手性化合物的比较
Comparative separation of chiral compounds by supercritical fluid chromatography and high performance liquid chromatography
摘要
Abstract
Supercritical fluid chromatographic( SFC)technique has been widely applied in the analysis,determinations and chromatographic preparations of chiral compounds in past decades due to some advantages such as fast speed,high efficiency and low consumption of organic sol-vents. Herein,24 kinds of chiral compounds(H1-H24)have been resolved on four polysaccha-ride-coated chiral stationary phases( CSPs)by SFC and high performance liquid chromatogra-phy( HPLC ),separately. Taking into account their retention times and selection factors of these compounds,SFC separation showed a better efficiency than HPLC for most of the com-pounds,except for some axis-chiral compounds. Some complementarity between SFC and HPLC separations was observed. For H15-H17,their retentions in SFC separations showed a strengthening trend with increasing carbon numbers of side alkyl chain,whereas a reverse tend-ency in HPLC separation was displayed. Most interestingly,an elution order reversal of metcon-azole enantiomers was found in this work. In brief,these results would provide some valuable references for the development of SFC separation methods in the future.关键词
超临界流体色谱/高效液相色谱/多糖手性固定相/手性分离/洗脱顺序反转/对映体Key words
supercritical fluid chromatography(SFC)/high performance liquid chromatogra-phy(HPLC)/polysaccharide-based chiral stationary phases/enantioseparation/elution order reversal/enantiomers分类
化学化工引用本文复制引用
张晶,陈晓东,李丽群,贺建峰,范军,章伟光..超临界流体色谱与高效液相色谱分离手性化合物的比较[J].色谱,2016,34(3):321-326,6.基金项目
国家自然科学基金项目（21171059，21571070）；科技型中小企业技术创新基金项目（13C26214404534）；广东省科技计划项目（2012B010900043，2014A010101145）；广州市科技计划项目（2013J4400027，201508020093）．@@@@Project of National Natural Science Foundation of China（Nos．21171059，21571070）；Innovation Fund for Technology Based Firms（ No．13C26214404534）；Guangdong Provincial Science and Technology Project （ Nos．2012B010900043，2014A010101145）； Guangzhou Science and Technology Project （ Nos．2013J4400027，201508020093）． （）
