色谱2016，Vol.34Issue(3)：351-355,5.DOI:10.3724/SP.J.1123.2015.11013
正相液相色谱法测定婴幼儿配方乳粉中1，3-二油酸-2-棕榈酸甘油三酯
Determination of 1,3-dioleoyl-2-palmitoyl triglyceride in infant formula milk powder by normal-phase liquid chromatography
宋戈 1亢美娟 1杨金宝1
作者信息
- 1. 东北农业大学，国家乳制品质量监督检验中心，黑龙江 哈尔滨 150028
- 折叠
摘要
Abstract
Methods for the determination of 1,3-dioleoyl-2-palmitoyl triglyceride ( OPO ) in infant formula milk powder by normal-phase liquid chromatography ( NP-LC ) and reversed-phase liquid chromatography( RP-LC ) were established and compared. The analytes in the infant formula were extracted by ethyl ether and petroleum ether,enriched by a rotary evapora-tor,and separated on silver-ion column and C18 column and detected with an evaporative light scattering detector( ELSD). OPO and 1,2-dioleoyl-3-palmitoyl triglyceride( OOP)can be basi-cally separated on a silver-ion column with a mixed solution of dichloromethane-acetone as mobile phase by gradient elution. The recoveries were 93. 0%-101. 5%,the relative standard deviations( RSD)were 2. 89%-4. 56%,and the limit of detection was 20 mg/kg. This method is accurate and stable for the determination of OPO in the infant formula milk powder.关键词
正相液相色谱/反相液相色谱/1,3-二油酸-2-棕榈酸甘油三酯/婴幼儿配方乳粉Key words
normal-phase liquid chromatography(NP-LC)/reversed-phase liquid chromatog-raphy(RP-LC)/1,3-dioleoyl-2-palmitoyl triglyceride(OPO)/infant formula milk powder分类
化学化工引用本文复制引用
宋戈,亢美娟,杨金宝..正相液相色谱法测定婴幼儿配方乳粉中1，3-二油酸-2-棕榈酸甘油三酯[J].色谱,2016,34(3):351-355,5.
