生物加工过程2016，Vol.14Issue(2)：1-6,6.DOI:10.3969/j.issn.1672-3678.2016.02.001
通过透明颤菌血红蛋白基因表达提高γ聚谷氨酸的生物合成
Expression of Vitreoscilla hemoglobin gene to enhance poly-γ-glutamic acid biosynthesis in Bacillus subtilis NX-2
摘要
Abstract
Biosynthesis of poly⁃γ⁃glutamic acid is an aerobic process. Therefore we tried to transform the strain by genetic engineering to improve its ability of using oxygen. First,the recombinant plasmid pMA5⁃P43⁃vgb was constructed with the overlapping PCR method connecting Bacillus subtilis P43 promoter to Vitreoscilla hemoglobin gene vgb, then transformed into Bacillus subtilis NX⁃2, to obtain a recombinant strain B.subtilis NX⁃2(vgb+).The molecular size of hemoglobin VHb in recombinant strain B. subtilis NX⁃2( vgb+) was 1�6 × 104 by SDS⁃PAGE. Further, the carbon monoxide difference spectra verified that the target protein had a physiological activity in B. subtilis NX⁃2 ( vgb+) . The experiments in the 7�5⁃L fermentor showed that the recombinant strain increased the biomass 41�2% and the γ⁃PGA production 7�5% than the original strain. The research lay a foundation for the γ⁃PGA industrial production.关键词
Bacillus subtilis/透明颤菌血红蛋白/γ聚谷氨酸Key words
Bacillus subtilis/Vitreoscilla hemoglobin/poly-γ-glutamic acid分类
通用工业技术引用本文复制引用
汤宝,冯小海,张丹,许宗奇,姜永翔,李莎,徐虹..通过透明颤菌血红蛋白基因表达提高γ聚谷氨酸的生物合成[J].生物加工过程,2016,14(2):1-6,6.基金项目
国家高技术研究发展计划（863计划）重大项目（2013AA020301）；普通高校研究生科研创新计划项目 （）
