生物加工过程2016，Vol.14Issue(2)：7-11,5.DOI:10.3969/j.issn.1672-3678.2016.02.002
重组大肠杆菌转化富马酸生产L丙氨酸
Transformation of fumaric acid for the production of L-alanine by recombinant Escherichia coli
摘要
Abstract
The gene coding for L⁃aspartate⁃β⁃decarboxylase from Comamonas testosteroni was cloned and expressed in an Escherichia coli strain CICC 11022S with the capability for L⁃aspartic acid production. The recombinant strain was used for the synthesis of L⁃alanine. The recombinant strain produced 112.7 g/L L⁃alanine from fumaric acid with a rate of 12.5 g/( L·h) and a conversion ratio of 93.8%. Fumaric acid has a low price,which efficiently reduces the substrate cost for L⁃alanine production. L⁃alanine was producd from fumaric acid by using the recombinant strain,and the results showed that the technology had industrial application potential.关键词
L丙氨酸/L天冬氨酸酶/L天冬氨酸β脱羧酶/重组工程菌Key words
L-alanine/L-aspartase/L-aspartate-β-decarboxylase/recombinant strain分类
生物科学引用本文复制引用
徐友强,马玉岳,姚粟,姜增妍,张奇,张露,裴疆森,程池..重组大肠杆菌转化富马酸生产L丙氨酸[J].生物加工过程,2016,14(2):7-11,5.基金项目
烟台市科技发展计划（2014SF151）；中国食品发酵工业研究院2015年科技发展基金（博士专项） （）
