生物加工过程2016，Vol.14Issue(2)：22-26,5.DOI:10.3969/j.issn.1672-3678.2016.02.005
磷脂酶D高产菌株的原生质体紫外诱变选育
Breeding of high phospholipase D-producing strain through mutation of protoplasts induced by UV radiation
摘要
Abstract
In order to screen a high⁃yielding phospholipase D strain from Streptomyces sp. LD0501, we studied the conditions for its protoplasts′preparation and regeneration, and mutagenized its protoplasts by UV radiation as well. The protoplasts were prepared by enzyme and mutagenized with UV irradiation. The mutation result was determined by TLC. The optimum conditions for the preparation of the protoplasts were as follows:the Streptomyces LD0501 was inoculated into mycelia medium with 0�5% glycine and cultured for 72 h�The mycelia were processed with 3 mg/mL lysozyme at 30℃ for 75 min. Protoplasts of Streptomyces sp. LD0501 were induced by UV radiation to obtain a high phospholipase D⁃producing strain,whose phospholipase D′s enzyme activity reached 4�29 U/mL, increased by 180�4% compared with the initial strain. The method improved the preparation effect of protoplasts from Streptomyces LD 0501 effectively and enhanced phospholipase D strain′s vitality greatly by UV irradiation of the protoplasts,the production of the high⁃yielding mutant strain was verified to be genetically stable.关键词
磷脂酶D/原生质体/紫外诱变/筛选Key words
phospholipase D/protoplast/UV mutagenesis/screening分类
生物科学引用本文复制引用
杨兰兰,张小里,封娟娟,朱南南,赵彬侠..磷脂酶D高产菌株的原生质体紫外诱变选育[J].生物加工过程,2016,14(2):22-26,5.基金项目
陕西省自然科学基础研究计划 （）
