生物加工过程2016，Vol.14Issue(2)：27-32,6.DOI:10.3969/j.issn.1672-3678.2016.02.006
一株产ε聚赖氨酸的白色链霉菌遗传转化体系
Genetic transformation system of a ε-poly-l-lysine-producing Streptomyces albulus strain
摘要
Abstract
To study the biosynthetic mechanism of ε⁃Poly⁃l⁃lysine (ε⁃PL ) in the producing strain, we developed a genetic transformation system for Streptomyces albulus PD⁃1,a well⁃known ε⁃PL⁃producing strain. Plasmid pIB139 was introduced into S. albulus PD⁃1 when Escherichia coli ET12567( pUZ8002) was used as a donor. After optimizing for conjugal transfer conditions,a maximal conjugation frequency of (3�0±0�4) × 10⁃6 per recipient was obtained. Further test indicated that plasmid pIB139 was integrated into the attB site of S. albulus PD⁃1 chromosome,and the integration of plasmid pIB139 was stable after passing several generations. This inter⁃generic conjugal transformation system for S. albulus PD⁃1 will provide powerful tools to study ε⁃PL biosynthesis mechanism and to construct high ε⁃PL over⁃producers.关键词
ε聚赖氨酸/白色链霉菌/遗传转化体系/pIB139Key words
ε-poly-l-lysine/Streptomyces albulus/genetic transformation system/pIB139分类
生物科学引用本文复制引用
孙朱贞,冯小海,许召贤,曹长虹,徐铮,徐虹..一株产ε聚赖氨酸的白色链霉菌遗传转化体系[J].生物加工过程,2016,14(2):27-32,6.基金项目
国家自然科学基金（21476112）；江苏省自然科学基金（BK20140933）；材料化学工程国家重点实验室自主课题 （）
