生物加工过程2016，Vol.14Issue(2)：47-50,4.DOI:10.3969/j.issn.1672-3678.2016.02.009
M.hydrocarbonoclasticus NY4中N2 OR的酶活测定及环境因素对酶活力的影响
Determination and characterization of nitrous oxide reductase in Mourinobacter hydrocarbonoclasticus NY4
摘要
Abstract
Nitrous oxide ( N2 O) is a major greenhouse gas,and its biological reduction has become a hot topic. Nitrous oxide reductase ( N2 OR ) is the only enzyme that can reduce N2 O to N2 in natural environments. Marinobacter hydrocarbonoclasticus NY4,a halophilic bacterium with the ability of anaerobic and aerobic denitrification,was used in this study�We established a stable method to determine the activity of N2 OR,and studied the influence of key environment factors on the activity of N2 OR including pH value, salt concentration and carbon/nitrogen ratio ( C/N) . When pH was at 7,the salinity was 6% and C/N was 8,strain NY⁃4 exhibited the highest N2 OR activity. The experimental data enriched the knowledge of the characteristics of N2 OR,and provided some references for the biological reduction of N2 O.关键词
pH/盐浓度/C/N/N2 ORKey words
pH/salinity/C/N/N2 OR分类
资源环境引用本文复制引用
王鹏胤,高远,高浩峰,胡南..M.hydrocarbonoclasticus NY4中N2 OR的酶活测定及环境因素对酶活力的影响[J].生物加工过程,2016,14(2):47-50,4.基金项目
国家自然科学基金 （）
