生物加工过程2016，Vol.14Issue(2)：51-57,7.DOI:10.3969/j.issn.1672-3678.2016.02.010
Leu1p参与调节VATP酶的活性
Leu1p involved in regulating V-ATPase activity
摘要
Abstract
RAVE ( regulator of the H+⁃ATPase of the vacuolar and endosomal membrane ) complex containing Rav1p, Rav2p and Skp1p, is a key regulatory factor of V⁃ATPase activity regulating mechanism. Latest news showed Leu1p capable of interacting with RAVE. This study,we constructed 4 Saccharomyces cerevisiae BY4742 recombinant strains that were lack of Rav1, Rav2, Vma2 and Leu1 respectively by fusion PCR and homologous recombination. RAVE for V⁃ATPase activity regulation is likely to be achieved by conjunction with Leu1p. Our study provided an important basis for studying the relationship between RAVE and Leu1p,also the study of regulation mechanism of the V⁃ATPase.关键词
VATP酶/RAVE/Leu1p/同源重组/融合PCRKey words
V-ATPase/RAVE/Leu1p/homologous recombination/fusion PCR分类
生物科学引用本文复制引用
高堂杰,张震宇..Leu1p参与调节VATP酶的活性[J].生物加工过程,2016,14(2):51-57,7.基金项目
国家自然科学基金 （）
