生物信息学2016，Vol.14Issue(1)：13-18,6.DOI:10.3969/j.issn.1672-5565.2016.01.03
台东苏铁中叶绿体功能基因RNA编辑的分析
Analysis of RNA editing sites in the chloroplast genome of Cycas taitungensis
沈成才1
作者信息
- 1. 北京师范大学克拉玛依附属学校，新疆 克拉玛依834000
- 折叠
摘要
Abstract
Cycas taitungensis is ancient gymnosperms, and the research of RNA editing in Cycas is limited. We analyzed the composition and distribution of RNA editing in Cycas taitungensis, which provided the useful information for exporing the function and mechanism of RNA editing,as well as for revealing the origin and evolution of higher plants.The thiocyanate method is appropriate for the RNA extraction of Cycas taitungensis. The cDNA was obtained by reverse transcribed the DNA⁃free RNA which treated with DNase.The RNA editing sites were found by RT⁃PCR,sequence and blast DNA and cDNA. Our results showed that the conversion of C⁃to⁃U and C⁃to⁃U editing events could be found in the ndhD2 and PetB transcripts of Cycas taitungensis as well as in ndhB2 transcript. We found that ndh genes have a higher rate of editing, while the serine codon was more frequently edited than the others.The results showed that there is a partially editing sites in ndhB2 transcrip,and silence editing sites also could be found in the ndhA2 transcript.关键词
台东苏铁/RNA编辑/RNA 提取/异硫氰酸胍法Key words
Cycas taitungensis/RNA editing/RNA extraction/Guanidine thiocyanate分类
生物科学引用本文复制引用
沈成才..台东苏铁中叶绿体功能基因RNA编辑的分析[J].生物信息学,2016,14(1):13-18,6.
