实验科学与技术2016，Vol.14Issue(1)：8-10,22,4.DOI:10.3969/j.issn.1672-4550.2016.01.003
正交设计太白贝母粗多糖的不同提取工艺
Research of Different Extraction Process of Polysaccharide From TaiBai Fritillariae with Orthogonal Test
摘要
Abstract
To study the extraction process of polysaccharide in TaiBai Fritillariae(PFT),the polysaccharide was extracted by wa-ter bath,ultrasonic and boiling method,and the content was assayed by enthrone colorimetric.Using orthogonal experiment analysis, the optimal extraction conditions of three extraction process were as follows:the water bath extraction method:temperature 70 ℃,solid-liquid ratio 1∶15,and extraction time 1 h,content of the optimum conditions of extraction of polysaccharide was 0.65 mg/g;The ultrasonic cell crushing extraction method:temperature 80 ℃,solid -liquid ratio 1∶25,and extraction time 15 min,content of the optimum conditions of extraction of polysaccharide was 0.99 mg/g;The boiling method:temperature 90 ℃,solid -liquid ratio 1∶20, and extraction time 3 h,content of the optimum conditions of extraction of polysaccharide was 0.38 mg/g.关键词
关/键/词/太白贝母粗多糖/正交设计/提取工艺Key words
TaiBai Fritillariae polysaccharide/orthogonal test/extraction process分类
轻工纺织引用本文复制引用
王红博,唐芳,刘美丽,陈林..正交设计太白贝母粗多糖的不同提取工艺[J].实验科学与技术,2016,14(1):8-10,22,4.基金项目
重庆市基础与前沿研究计划项目（CSTC2013JCYJA10120）；2014年重庆三峡学院科学研究青年资助项目。 （）
