中国病理生理杂志2016，Vol.32Issue(3)：392-397,6.DOI:10.3969/j.issn.1000-4718.2016.03.002
JAK／STAT 信号通路在高糖诱导人脐静脉内皮细胞损伤中的作用
Role of JAK/STAT signaling pathway in high glucose-induced damage in human umbilical vein endothelial cells
摘要
Abstract
AIM: To investigate whether Janus kinase/signal transducer and activator of transcription ( JAK/STAT) signaling pathway mediates high glucose-induced damage in human umbilical vein endothelial cells ( HUVECs ) . METHODS:The cell viability was examined by CCK-8 assay.The expression levels of JAK2, STAT3, caspase-9 and en-dothelial nitric oxide synthase ( eNOS) were detected by Western blot .The intracellular levels of reactive oxygen species ( ROS) were tested by DCFH-DA staining followed by photofluorography .Mitochondrial membrane potential ( MMP) was measured by rhodamine 123 staining followed by photofluorography .RESULTS:Treatment of HUVECs with high glucose (40 mmol/L glucose) for 6~12 h enhanced the protein level of phosphorylated JAK 2, peaking at 9 h.Treatment of the cells with high glucose for 6~12 h also increased the protein level of p-STAT3 with the peak value at 12 h.Pretreatment with the inhibitor of JAK/STAT pathway AG490 for 1 h before exposure of the HUVECs to high glucose significantly inhibi-ted the high glucose -induced injury , as evidenced by an increase in the cell viability , decreases in the expression of caspase-9 and the intracellular ROS production , and increases in MMP and the expression of eNOS .CONCLUSION:JAK/STAT signaling pathway is involved in the high glucose-induced damage in HUVECs .关键词
JAK/STAT信号通路/高糖/人脐静脉内皮细胞/AG490Key words
JAK/STAT signaling pathway/High glucose/Human umbilical vein endothelial cells/AG490分类
医药卫生引用本文复制引用
廖静秋,林佳琼,张伟杰,许翎,智喜梅,林凯,吴文..JAK／STAT 信号通路在高糖诱导人脐静脉内皮细胞损伤中的作用[J].中国病理生理杂志,2016,32(3):392-397,6.基金项目
国家自然科学基金资助项目（No．81450062）；广东省自然科学基金资助项目 （）
