中国病理生理杂志2016，Vol.32Issue(3)：425-431,7.DOI:10.3969/j.issn.1000-4718.2016.03.007
上皮性卵巢癌中 PARP-1的表达及其与 EMT 的关系
Expression of PARP-1 in epithelial ovarian cancer and its relationship with epithelial-mesenchymal transition
摘要
Abstract
AIM:To investigate the expression of poly (ADP-ribose) polymerase-1 (PARP-1) in the epithelial ovarian cancer ( EOC) and its relationship with epithelial-mesenchymal transition ( EMT) .METHODS:The expression of PARP-1, E-cadherin, vimentin and Snail was detected in the EOC and benign ovarian tumor tissues by immunohistochemi -cal method and real-time PCR.The expression of PARP-1, E-cadherin, vimentin and Snail proteins in the SKOV3 cells treated with efficient PARP-1 inhibitor PJ34 was determined by Western blotting .RESULTS:The positive expression rates of PARP-1, vimentin and Snail were significantly higher in the EOC than that in the benign ovarian tumor tissues , whereas the positive expression rate of E-cadherin was the opposite (P<0.05).The expression of PARP-1, E-cadherin, vimentin and Snail in the EOC was associated with the histological grade , clinical stage and lymphatic metastasis (P<0.05), but no relationship with age and pathological types was observed .The expression of E-cadherin in the EOC was negatively co-related to that of PARP-1.In contrast, the expression of vimentin and Snail in the EOC was positively co-related to that of PARP-1.The relative mRNA expression of PARP-1, vimentin and Snail in the EOC was significantly higher than that in the benign ovarian tumor tissues ( P<0.05) , while the mRNA expression of E-cadherin in the EOC was remarkably lower than that in the benign ovarian tumor tissues (P<0.05).The protein expression of PARP-1, vimentin and Snail in the SKOV3 cells was significantly decreased (P<0.05), while E-cadherin protein was increased after treated with PJ 34(P<0.05). CONCLUSION:PARP-1 may contribute to the onset of EMT in the EOC by regulating the expression of E -cadherin, vim-entin and Snail.The role of PARP-1, which is relevant to EMT, might be important in the development of ovarian cancer .关键词
卵巢癌/聚腺苷二磷酸核糖聚合酶-1/E-钙黏蛋白/波形蛋白/Snail/上皮-间质转化Key words
Ovarian cancer/Poly (ADP-ribose) polymerase-1/E-cadherin/Vimentin/Snail/Epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
张灿灿,尉蔚,林雪艳,田永杰..上皮性卵巢癌中 PARP-1的表达及其与 EMT 的关系[J].中国病理生理杂志,2016,32(3):425-431,7.基金项目
国家自然科学基金应急管理项目（No．81441075）；山东省自然科学基金资助项目（No．ZR2014HM108）；山东省自然科学基金青年项目 （）
