中国病理生理杂志2016，Vol.32Issue(3)：522-526,533,6.DOI:10.3969/j.issn.1000-4718.2016.03.022
慢性心衰大鼠下丘脑室旁核瞬时外向钾通道蛋白Kv4.2和 Kv4.3低表达促进肾交感神经兴奋性
Downregulated transient outward potassium channel protein Kv4.2 and Kv4.3 expression in PVN contributes to sympathoexcitation in rats with chronic heart failure
摘要
Abstract
[ ABSTRACT] AIM:To investigate the transient outward potassium channel protein expression in paraventricular nucleus (PVN) and its contribution to renal sympathetic nerve activity (RSNA) in rats with chronic heart failure (CHF). METHODS:A rat model of CHF was prepared by acute myocardial infarction that was induced by ligation of the left ante -rior descending coronary artery .Four weeks after heart failure , echocardiogram was applied to identify the CHF model and plasma norepinephrine (NE), serum NH2-terminal pro-brain natriuretic peptide (NT-proBNP) were detected by ELISA. The expression of ransient outward potassium channel proteins Kv 4.2 and Kv4.3 at mRNA and protein levels was deter-mined by real-time PCR and Western blot .The mean arterial pressure ( MAP) , heart rate ( HR) and RSNA were measured in anesthetized rats with PVN microinjection of potassium channel blockers 4-AP.RESULTS:In CHF group , the rat car-diac function and Kv4.2 and Kv4.3 expression in PVN were obviously lower while plasma NE and serum NT-proBNP were obviously higher than those in sham group .Microinjection of 4-AP into PVN induced an increase in MAP , HR and RSNA in both sham and CHF rats , while the CHF rats exhibited smaller responses to 4-AP than sham-operated rats .CONCLU-SION:Downregulation of Kv4.2 and Kv4.3 expression in the PVN may be a potential mechanism for sympathoexciation in the rats with chronic heart failure .关键词
慢性心力衰竭/交感神经/下丘脑室旁核/瞬时外向钾通道蛋白Key words
Chronic heart failure/Sympathetic nervous/Hypothalamic paraventricular nucleus/Ransient out-ward potassium channel protein分类
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马柳一,尹玉洁,位庚,李红蓉,张军芳,刘焕,贾振华..慢性心衰大鼠下丘脑室旁核瞬时外向钾通道蛋白Kv4.2和 Kv4.3低表达促进肾交感神经兴奋性[J].中国病理生理杂志,2016,32(3):522-526,533,6.基金项目
国家重点基础研究发展计划（973计划）（No．2012CB518606）；国家自然科学基金资助项目 （）
