中国免疫学杂志2016，Vol.32Issue(4)：460-464,5.DOI:10.3969/j.issn.1000-484X.2016.04.002
miRNA-126基因敲减小鼠脾脏中免疫细胞的组成变化
Change on composition of immune cells in spleen of miRNA-126 knockdown mice
摘要
Abstract
Objective:To detect the change of composition of immune cells in the spleen of miR-126 knockdown(miR-126KD) mice and preliminarily explore its significance .Methods: The expression level of miR-126 in spleens of miR-126KD mice was deter-mined by Realtime PCR.And the total number of splenocytes was calculated.The pathologic morphology change of the spleen was observed by HE staining.And the changes on proportion of DCs ,macrophages cells ,γδ T cells and NK T cells,CD3+T cells and its subgroup ,as well as CD19+B cells in spleens of miR-126KD mice were analyzed by Flow cytometry and calculated respectively.The level of phosphorylated AKt and NF-κB was analyzed by Western blot assay.Results:Compared with those of WT mice ,the expression level of miR-126 decreased obviously ( P<0.05 ) and the total number of cells increased obviously in spleen of miR-126 KD mice ( P<0.05).Moreover,the pathologic morphology of miR-126KD mice was significantly changed.The proportion and number of NK T cells in the inherent cells were significantly increased ( P<0.05 ) , but the proportion of Mφcells were significantly decreased ( P<0.05 ) . Meanwhile,the proportion and number of CD3+T cells and CD4+T cells in the adaptive immune cells were significantly increased (P<0.05),while the total number of CD19+B cells were significantly decreased (P<0.05).Last,the level of phosphorylation Akt and NF-κB increased obviously in spleen of miRNA-126 knockdown mice.Conclusion: Change obviously on the composition of immune cells subsets in the spleen of miRNA-126 knockdown mice ,which it may be related to the altered level of phosphorylated AKt and NF-κB and provides a preliminary experimental basis for the further exploring the roles of miR -126 in immune response.关键词
miRNA-126/基因敲减/免疫细胞/组成Key words
miR-126/Knockdown/Immune cells/Composition分类
医药卫生引用本文复制引用
雷良玉,胡燕,郭萌萌,卢佳,郑文,徐华林,陈超,徐林..miRNA-126基因敲减小鼠脾脏中免疫细胞的组成变化[J].中国免疫学杂志,2016,32(4):460-464,5.基金项目
本文受国家自然科学基金（No．31370918）和教育部新世纪优秀人才计划（NCET-12-0661）资助。 （）
