中国免疫学杂志2016，Vol.32Issue(4)：509-512,4.DOI:10.3969/j.issn.1000-484X.2016.04.013
HSPC238对HMOX1、RPS27a、MT2A、UBB调节的初步研究
A preliminary study on regulation of HMOX1, RPS27a, MT2A and UBB by HSPC238
摘要
Abstract
Objective:To investigate the effect of HSPC 238 overexpression or low expression on the regulation of the target protein ( HMOX1, MT2A, UBB, RPS27a ) and the regulation pathways.Methods: To construct the interference vector and overexpression vector of HSPC238 respectively,transfected the HEPG2 cell lines by the liposome method,detect the expression of mRNA and protein of the HSPC 238 and the target proteins by the RT-PCR and Western blot , further to transfer the overexpression plasmids of the target proteins into the HEPG 2 cell lines which had been transfected with interference vector and overexpression vector of HSPC238,the experimental group cell lines were treated with proteasome inhibitor MG 132,to purify the target proteins with nickel column,do immunoblotting with HSPC238 to detect the accumulation situation of the target proteins.Results: The HMOX1,MT2A, RPS27a were downregulated obviously when the HSPC 238 was interfered exogenous;and the MT2A,UBB,RPS27a were up-regulated after the HSPC238 overexpressed.The overexpression plasmid of target proteins were transfected into the HEPG 2 cell lines which have been transfected with interference vector and overexpression vector of HSPC 238 ,compared with the control group ,the target protein band in the experimental group was significantly increased after treatment with the proteasome inhibitor MG 132.Conclusion:The HSPC238 overexpression can upregulate the expression of MT 2A,UBB and RPS27a,and interfering HSPC238 can downregulate the expression of HMOX1,MT2A and RPS27a;the HSPC238 target protein may play a regulatory role through the UPP pathway;the HSPC238 may play a regulatory role on the target proteins through the UPP pathway.关键词
HSPC238/泛素蛋白酶体途径/目标蛋白Key words
HSPC238/Ubiquitin-proteasome pathway/Target protein分类
医药卫生引用本文复制引用
谭家余,黄湘,陈敬林,钟裕恒..HSPC238对HMOX1、RPS27a、MT2A、UBB调节的初步研究[J].中国免疫学杂志,2016,32(4):509-512,4.基金项目
本文受国家自然科学基金（81101534）、广东省自然科学基金（S2012010010824）和广东省医学科研基金（A2013875）项目资助。 （）
