中国免疫学杂志2016，Vol.32Issue(4)：513-518,6.DOI:10.3969/j.issn.1000-484X.2016.04.014
MMS2和P53基因对结肠癌细胞增殖与凋亡的影响
Interaction between MMS2 and P53 on proliferation and apoptosis of colon cancer cells
摘要
Abstract
Objective:To explore the regulatory effects of proliferation and apoptosis on THC-8307 by MMS2 siRNA and P53 siRNA.Methods:We experimentally suppressed the MMS2 and P53 expression in human colon cancer cells by the interference RNA technology ( RNAi) as monitored by Real-time qRT-PCR and Western blot.THC-8307 cells that express rate significantly reduced were collected as case group , while using untreated cells as the blank control group , and mock-treated cells as the negative control group.After separately interfering the target genes in each group ,test the relationship and expression level of the two genes.Utilizing flow cytometry techniques to test cells proliferation and apoptosis rate of each group.Results: Compared to the control group , colon cancer cells in which MMS2 and P53 were silenced displayed significant increase of P53,MMS2 mRNA and protein levels(P<0.05).MMS2-depleted cells displayed increase in apoptosis rates ,for both early and later stages ( P<0.05 ).Conclusion: MMS2 and P53 negatively regulate each other in colon cancer cells proliferation and apoptosis .关键词
MMS2/P53/RNA干扰/人结肠癌细胞/细胞凋亡Key words
MMS2/P53/RNA-interference/Human colon cancer cells/Apoptosis分类
医药卫生引用本文复制引用
马琳园,隋御,马璐,李昕,李元杰,徐方..MMS2和P53基因对结肠癌细胞增殖与凋亡的影响[J].中国免疫学杂志,2016,32(4):513-518,6.基金项目
本文为国家自然科学基金资助项目（No.313602501）。 （）
