中国全科医学2016，Vol.19Issue(11)：1287-1291,5.DOI:10.3969/j.issn.1007-9572.2016.11.012
儿童注意缺陷多动障碍与5-羟色胺R2A基因多态性的相关性研究
Association of 5-HT R2A Gene Polymorphism and Attention Deficit Hyperactivity Disorder in Children
摘要
Abstract
Objective To investigate the association between 5-HT R2A gene single nucleotide polymorphisms and attention deficit hyperactivity disorder( ADHD)in children. Methods By random number table method,we enrolled 158 ADHD children as case group,from ADHD children found in the ADHD epidemiological sampling survey among children in Guangxi Province conducted from December 2011 to January 2013. We also enrolled 159 children without ADHD with matching genders and ages among normal population in the same residential areas of children with ADHD as control group. From each subject,5 ml peripheral venous blood was sampled,and DNA was extracted. According to the data about SNPs in the 5-HT R2A gene of Chinese provided by HapMap and relevant literature published at home and abroad,we selected 14 SNPs and designed primer sequence. After PCR amplification,genotypes were determined at the reaction endpoint. Results Case group and control group were significantly different in the genotype distribution of rs2770304,rs985934 and rs6311 locus;case group was higher in the frequency of CC/ CT genotype and lower in the frequency of TT genotype than control group( P < 0. 05). Case group was higher than control group in the frequency of alleles associated with rs2770304,rs985934,rs4941573,rs2070040,rs6313 and rs6311 locus(P < 0. 05). Strong linkage disequilibrium existed among rs1745837,rs3742279 and rs2296972 locus(D' and r2 were both larger than 0. 95 ),among rs2770304,rs985934 and rs4941573 locus,among rs2070040,rs6313 and rs6311 locus. Case group was higher in the haplotype frequency of CCA and ACC and lower in the frequency of haplotype TTG and GTT than control group( P < 0. 05). Conclusion The polymorphism of rs2770304,rs985934,rs4941573,rs2070040,rs6313 and rs6311 locus and haplotype CCA,ACC,TTG and GTT of 5-HT R2A gene may be correlated with ADHD in children.关键词
注意力缺陷障碍伴多动/5-羟色胺/多态性,单核苷酸/连锁不平衡Key words
Attention deficit disorder with hyperactivity/5 - Hydroxytryptamine/Polymorphism,single nucleotide/Linkage disequilibrium分类
医药卫生引用本文复制引用
梁友芳,农清清,覃健敏,韦宏旷,郭尧平,范誉,班彩霞,李慕军..儿童注意缺陷多动障碍与5-羟色胺R2A基因多态性的相关性研究[J].中国全科医学,2016,19(11):1287-1291,5.基金项目
国家自然科学基金资助项目（81360420）；广西自然科学基金面上项目（2011GXNSFA018294）；广西高等学校重点资助科研项目 （）
