中国药房2016，Vol.27Issue(10)：1315-1318,4.DOI:10.6039/j.issn.1001-0408.2016.10.06
盐酸椒苯酮胺对大鼠和Beagle犬的急性毒性实验研究
Experimental Study on Acute Toxicity of Piperphentonamine Hydrochloride in Rats and Beagle Dogs
摘要
Abstract
OBJECTIVE:To investigate acute toxicity of piperphentonamine hydrochloride (PPTA) in rats and Beagle dogs, and to calculate maximum dosage and evaluate its safety. METHODS:SD rats were given relevant medicine via ig or iv,at a sin-gle dose of 50 mg/kg,every 4-6 h,for consecutive 3 times,150 mg/kg in total;after 14 d treatment,the toxicity reaction and body weight of rats were observed in PPTA group (n=40) and solvent control group (n=20). Beagle dogs were given PPTA ivgtt,at maximal single dose of 200 mg/kg;after 14 d of treatment,Beagle dogs of PPTA group(n=4)and solvent control group (n=3) were observed in respects of general physiological index,body weight,body temperature,ECG index,blood pressure, plasma electrolyte,urine,ophthalmology and pathological index,etc. RESULTS:There was no obvious abnormality in rats after ig administration of PPTA. One-off action instability occurred after iv within minutes;no abnormality was found for 14 days;the increase of body weight of rats in treatment groups was normal. Transient conjunctival congestion,nausea and vomiting,saliva-tion,mild edema eyelids and rear limb weakness were observed in Beagle dogs during dosing;only one Beagle dog suffered from hematuria caused by hemorrhage of bladder. 14 d after medication,there was no statistical significance in body weight and tempera-ture of Beagle dogs between treatment groups and solvent control group(P>0.05). No abnormal changes due to drug toxicity reac-tion were observed. CONCLUSIONS:LD50 values of PPTA in rats via ig and iv are over 150 mg/kg. Beagle dogs did not died at the single dose of 200 mg/kg PPTA,ivgtt. The abnormal signs of animals may be the side effects of PPTA. The main toxic reaction of PPTA may be hemorrhage of bladder and other.关键词
盐酸椒苯酮胺/急性毒性/大鼠/Beagle犬/最大给药量Key words
Piperphentonamine hydrochloride/Acute toxicity/Rats/Beagle dogs/Maximal dose分类
医药卫生引用本文复制引用
李健,李茹冰,张强,陈新,尹雁,朱晋辉,樊鸿浩,邓乐君,付岩霖..盐酸椒苯酮胺对大鼠和Beagle犬的急性毒性实验研究[J].中国药房,2016,27(10):1315-1318,4.基金项目
广东省教育部产学研结合项目(No. 2012B091100-170) （No. 2012B091100-170）
广东省科技计划项目(广东省重大科技专项)(No. 2012A08020-4010) （广东省重大科技专项）
广州市科技计划项目(No. 201509010012) （No. 201509010012）
