中国药房2016，Vol.27Issue(10)：1318-1321,4.DOI:10.6039/j.issn.1001-0408.2016.10.07
梓醇对成骨细胞-破骨细胞共育体系中破骨细胞活性、凋亡的影响及其机制研究
Effect of Catalpol on the Activity and Apoptosis of Osteoclast in the Osteoblasts-osteoclasts Co-culture Sys-tem and Its Mechanism Study
摘要
Abstract
OBJECTIVE:To study the effect of catalpol on the activity and apoptosis of osteoclasts (OC) in the osteoblasts (OB)-OC co-culture system and its mechanism. METHODS:OB and OC were isolated respectively from the SD rats of 1-3 days and 5-7 days old to establish OB-OC co-culture system. After treated with 0(blank control),0.05,0.5,5,50 and 100 mg/L catal-pol for 48,72 and 96 h,the number of bone absorption lacuna for OC was observed by inverted microscope to reflect OC activity. After treated with 0(blank control)and 0.05 mg/L catalpol for 48,72 and 96 h,the activity of tartrate resistant acid phosphatase (TRACP)in OC was detected,and the apoptosis rate of OC was calculated. After treated with 0(blank control)and 0.05 mg/L ca-talpol,mRNA expression of osteoprotegerin(OPG)in OB was detected. RESULTS:In OB-OC co-culture system,the number of bone absorption lacuna in 0.05-50 mg/L catalpol groups was significantly lower than blank control group(P<0.01),indicating ca-talpol could inhibit OC activity,especially 0.05 mg/L catapol. Compared with blank control,0.05 mg/L catapol lowered the activity of TRACP but increased the apoptosis rate of OC(P<0.05);mRNA expression of OPG was up-regulated in OB(P<0.01). CON-CLUSIONS:In OB-OC co-culture system,catalpol can inhibit the activity of OC and induce the apoptosis of OC,and its mecha-nism may be associated with the mRNA expression up-regulation of OPG in OB.关键词
梓醇/成骨细胞/破骨细胞/共育体系/活性/凋亡Key words
Catalpol/Osteoblasts/Osteoclasts/Co-culture system/Acitivty/Apoptosis分类
医药卫生引用本文复制引用
赖满香,陈侠,冯娟,何丽霞,杨丽..梓醇对成骨细胞-破骨细胞共育体系中破骨细胞活性、凋亡的影响及其机制研究[J].中国药房,2016,27(10):1318-1321,4.基金项目
广东省医学科学技术研究基金项目(No.B2015063) （No.B2015063）
广东食品药品职业学院自然科学基金项目(No.2014YZ003) （No.2014YZ003）
