中国药房2016，Vol.27Issue(10)：1329-1332,4.DOI:10.6039/j.issn.1001-0408.2016.10.10
蒙药肋柱花水提物对D-GlaN和CCl4致小鼠急性肝损伤的保护作用研究
Study on Protective Effect of Mongolian Medicine Lomatogonium rotatum Water Extract against Acute Liv-er Injury in Mice Induced by D-GlaN and CCl4
摘要
Abstract
OBJECTIVE:To study the protective effects of Mongolian medicine Lomatogonium rotatum water extract against acute liver injury in mice caused by D-GlaN and CCl4. METHODS:60 mice were randomly divided into normal group(normal sa-line),D-GlaN model group(normal saline),positive control group(Kuihua hugan tablet,0.56 g/kg)and L. rotatum water extract high-dose,medium-dose and low-dose groups [3,1.5,0.75 g(crude drug)/kg] 10 rats in each group. They were given relevant medicine intragastrically once a day for consecutive 23 days. 30 min after last administration,those group were given D-GlaN ip to induce acute liver injury model except normal group. The activity of AST,ALT,ALP,and acetylcholinesterase(CHE)content in serum were detected,and liver index was calculated. Liver histopathology was observed and scored. Other 60 mice were selected, and then grouped,given medicine and detected in lab index with same method with above group,except for modeling method(ig, 0.1% CCl4). RESULTS:Compared with normal group,necrosis or degeneration of liver cells were obvious in 2 model groups, pathological score,the activity of AST,ALT and ALP increased while CHE content decreased;liver index of D-GlaN model group increased while that of CCl4 model group decreased (P<0.05). Compared with D-GlaN model group,the activity of AST,ALT and ALP and liver index decreased in L. rotatum water extract groups,while CHE content increased(P<0.05);pathological mor-phology had been improved,and pathological score decreased. Compared with CCl4 model group,ALT activity decreased in L. rota-tum water extract low-dose group,while CHE content and liver index increased;CHE content of L. rotatum water extract medi-um-dose group decreased,while liver index increased;the activity of AST,ALT and ALP decreased in L. rotatum water extract high-dose group,while CHE content increased(P<0.05). Pathological morphology of L. rotatum water extract groups had been im-proved to certain extent,and pathological score decreased. CONCLUSIONS:L. rotatum water extract could protect acute liver inju-ry in mice induced by D-GlaN and CCl4.关键词
蒙药/肋柱花水提物/D-氨基半乳糖胺/四氯化碳/急性肝损伤/保护作用/小鼠Key words
Mongolian medicine/Lomatogonium rotatum water extract/D-GlaN/CCl4/Acute liver injury/Protective effect/Mice分类
医药卫生引用本文复制引用
包明兰,巴根那,辛颖,白梅荣,何那拉..蒙药肋柱花水提物对D-GlaN和CCl4致小鼠急性肝损伤的保护作用研究[J].中国药房,2016,27(10):1329-1332,4.基金项目
内蒙古自治区自然科学基金资助项目(No.2014MS-0830) （No.2014MS-0830）
内蒙古民族大学科学研究项目(No.NMDGP1506) （No.NMDGP1506）
内蒙古民族大学博士科研启动基金项目(No.BS357) （No.BS357）
内蒙古民族大学2014年度博士、硕士研究生科研创新项目(No.NMDSS1445) （No.NMDSS1445）
