中国卒中杂志2016，Vol.11Issue(4)：269-276,8.DOI:10.3969/j.issn.1673-5765.2016.04.006
HDAC9基因SNPs与大动脉粥样硬化缺血性卒中的相关性研究
Study on the Correlation between HDAC9 Gene SNPs and Large Vessel Atherosclerosis-induced Ischemic Stroke
柳维林 1林云娇 2陶静 1彭军 3李光谱 3陈立典4
作者信息
- 1. 350122 福州福建中医药大学康复医学院
- 2. 福建省运动功能康复重点实验室
- 3. 福建中西医结合研究院
- 4. 美国俄克拉荷马州立大学健康医学中心
- 折叠
摘要
Abstract
Objective To investigate the correlation between histone deacetylase 9 (HDAC9) gene susceptible single nucleotide polymorphisms (SNPs) and genotype and its phenotype in large vessel atherosclerosis-induced ischemic stroke. <br> Methods By using the Haploview software and data on genetics from thousands of human genome project (HapMap) , the analysis of SNPs of HDAC9 gene, minor allele frequency (MAF), single block and haplotype and tag SNPs were performed. <br> Results A total of 75 SNPs in the Chr7:18930kb--19020kb regions of HDAC9 gene were determined, of which 51 SNPs accorded with Hardy-Weinberg balance (P>0.05), and MAF (P>0.05). Based on the conifdence intervals, four Gamete rule and solid spine of LD, the 11, 14 and 8 single domains were constructed, respectively. Among which, the rs2717356 site was low linkage disequilibrium (LD) and outside of the single domain belonging to HDAC9 gene recombination hotspots. A total of 30 SNPs were determined as haplotype tagging SNPs (htSNPs) according with R2 was more than or equal to 0.8, and LOD was more than or equal to 3.0, and that showed MAF was more than 0.10. The rs2526630 [C/T] site located in HDAC9 3 'UTR region, binding with microRNAs like hsa-miR-545, hsa-miR-616, etc. <br> Conclusion The 30 htSNPs served as reference for HDAC9 gene susceptibility SNP sites for screening and validating repeatedly. The binding of rs2526630 site of HDAC9 gene and miRNA might associate with the morbidity of large vessel atherosclerosis-induced ischemic stroke.关键词
HDAC9基因/SNPs/缺血性卒中Key words
HDAC9 gene/SNPs/Ischemic stroke引用本文复制引用
柳维林,林云娇,陶静,彭军,李光谱,陈立典..HDAC9基因SNPs与大动脉粥样硬化缺血性卒中的相关性研究[J].中国卒中杂志,2016,11(4):269-276,8.
